医学信息Issue(48)：127-128,2.
大鼠二次脑挫伤模型的建立
Rat Brain Contusion Model with Twice Traumatic Brain Injuries
董国凯 1薛宏涛 2谢华 3孙晓明 1殷文江 1李周儒1
作者信息
- 1. 徐州医学院法医学教研室 江苏 徐州 221002
- 2. 徐州市铜山区公安局刑警大队 江苏 徐州 221100
- 3. 新沂市公安局刑警大队 江苏 徐州 221400
- 折叠
摘要
Abstract
Objective To establish a brand new rat model for researches after twice traumatic brain injuries in forensic investigation. Methods Mature SD rats were classified into 3 groups randomly, A:sham-operated group, B:brain injury once, and C:brain injury twice, without restriction of gender. The injuries were introduced using a free fal device to the left side of the brain of rats in group B and C. 1 hour later, the same injure was introduced to the other side of the brain of rats in group C. Samples were taken at 0h, 1h, 2h after injury for group B. For group C, both sides were sampled 1h after the second injury. The expression of C-FOS protein was tested as indicator for injury with laser scanning confocal microscope together with the computer colorful image analysis technique. Results The C-FOS expressed dif erently in the pan-injury areas between group B and C at dif erent time point ( <0.05). Dif erences were also found between the pan-area of the first and second injury in Group C ( <0.05). Conclusion This model could be used in the studies for the secondary injury. It could also help investigate the exits of the secondary injury.关键词
法医病理学/脑挫伤/C-FOS/动物模型Key words
Forensic pathology/Brain injury/C-COS/Animal model引用本文复制引用
董国凯,薛宏涛,谢华,孙晓明,殷文江,李周儒..大鼠二次脑挫伤模型的建立[J].医学信息,2015,(48):127-128,2.
