中国病理生理杂志Issue(12)：2113-2119,7.DOI:10.3969/j.issn.1000-4718.2015.12.001
敲低 IK1钾通道抑制 ClC－3氯通道的表达和功能
Knockdown of IK1 potassium channel inhibits expression and function of ClC-3 chloride channel
摘要
Abstract
[ ABSTRACT] AIM:To investigate whether the ClC-3 chloride channel is an acting target of the IK 1 potassium channel, and to study the action of IK1 potassium channel on the functional activities and expression of ClC-3 chloride channels.METHODS: IK1 gene was silenced by IK1 siRNA in poorly-differentiated nasopharyngeal carcinoma cells (CNE-2Z).Real-time PCR and Western blot were used to detect the expression of ClC-3 at mRNA and protein levels.The distribution of ClC-3 protein in the cells was observed under confocal immunofluorescence microscope .The chloride current was recorded by the patch-clamp technique.RESULTS: IK1 siRNA was successfully transfected into the CNE-2Z cells and knocked down the expression of IK 1 potassium.The mRNA expression of ClC-3 was increased by the IK1 siRNA.IK1 siRNA inhibited the expression of ClC-3 protein.A chloride current was activated by hypotonic challenges , and the hypoto-nicity-induced current was reduced in the cells which successfully transfected with IK 1 siRNA.CONCLUSION: The knockdown of IK 1 potassium channels inhibits the expression and function of ClC-3 chloride channel .关键词
ClC-3氯通道/IK1钾通道/鼻咽癌/膜片钳技术Key words
ClC-3 chloride channel/IK1 potassium channel/Nasopharyngeal carcinoma/Patch-clamp tech-niques分类
医药卫生引用本文复制引用
邹丽莉,叶东,吕瑞玲,汪源,孙晓雪,朱林燕,陈丽新,王立伟..敲低 IK1钾通道抑制 ClC－3氯通道的表达和功能[J].中国病理生理杂志,2015,(12):2113-2119,7.基金项目
国家自然科学基金资助项目 （）
