中国病理生理杂志Issue(12)：2259-2264,6.DOI:10.3969/j.issn.1000-4718.2015.12.025
体外诱导人肾间质成纤维细胞成骨分化
Induction of osteogenic differentiation of human renal fibroblasts in vitro
摘要
Abstract
AIM:To investigate the effects of osteogenic induction media and the medias containing different concentration of calcium on the induction of osteogenic differentiation of human renal fibroblasts in vitro.METHODS: Culturedhuman renal fibroblasts were divided into 5 groups in this experiment: osteogenic induction group (osteogenic inductionmedia), CaⅠgroup (0.5 mmol/L Ca2 + media), CaⅡgroup (1.5 mmol/L Ca2 + media), Ca Ⅲ group (2.5 mmol/LCa2 + media) and control group (PBS).The cell activity in each groups was measured by MTT assay .At 9th day, the cellcalcium Alizarin red S staining and alkaline phosphatase (ALP) Gomori calcium cobalt staining were performed respectivelyto observe the formation of calcium nidus and the expression of ALP .In addition, the expression of Runt-related transcriptionfactor 2 (Runx2) at mRNA and protein levels was determined by real -time PCR and Western blot, respectively.RE- SULTS: The remarkable positive signs which represented the formation of calcium nidus and the deposit of calcium objectsin all experiment groups were observed .The mRNA and protein expression of Runx2 in osteogenic induction group increasedin accordance with the induction time .Compared with control group, the mRNA and protein expression of Runx2 inthe CaⅠ ~Ⅲ groups increased gradually in a calcium concentration dependent manner at the 9th induction day.CON- CLUSION: Human renal interstitial fibroblasts show the potential activity in osteogenic differentiation induced by osteogen -ic induction media or high level calcium in vitro, which may be account for the cytological formation of the Randall ’splaque in the kidney.关键词
人肾成纤维细胞/成骨分化/肾乳头钙化斑/高钙尿症/肾结石Key words
Human renal interstitial fibroblasts/Osteogenic differentiation/Randall’s plaque/Hypercalci-uria/Renal calculi分类
医药卫生引用本文复制引用
严一杰,黎承杨,邓耀良,曾国华,陶芝伟,刘云龙,孙春,王扬..体外诱导人肾间质成纤维细胞成骨分化[J].中国病理生理杂志,2015,(12):2259-2264,6.基金项目
广西自然科学基金资助项目（No．2011GXNSFA018177）；广东省泌尿外科重点实验室（No．2010A060801016）资助 （）
