5株水貂阿留申病毒的分离与鉴定OA
Isolation and Identiifcation of Five Strains of Aleutian DiseaseViruses in Mink
为给水貂阿留申病疫苗的研制奠定基础，对疑似感染阿留申病死亡的水貂内脏处理后，接种猫肾传代细胞（CRFK）分离病毒，并对分离的毒株进行PCR鉴定及动物回归试验。与此同时，首次应用免疫过氧化物酶单层细胞实验（IPMA）对所分离的水貂阿留申病毒进行TCID50的测定。结果成功分离并鉴定出5株水貂阿留申病毒株，分别命名为ADV-DL124、ADV-DL125、ADV-ZJ3、ADV-QD2、ADV-QD3，各分离株的TCID50分别为105.7TCID5…查看全部>>
In order to lay foundation for the development of Aleutian disease vaccine,the CRFK cells were used to iso-late the ADV with viscera samples collected from the dead minks which was suspected ADV infection sampled. The isolated strains were identified by PCR and regression experiments on animal. At the same time,Immunoperoxidase monolayer assay(IPMA)was used to detect the median tissue culture infective doses(TCID50)of isolated strains in the first time. Five…查看全部>>
刘东旭;时坤;李健明;曾范利;刘菲;杜锐
吉林农业大学中药材学院，吉林长春 130118吉林农业大学中药材学院，吉林长春 130118吉林农业大学中药材学院，吉林长春 130118吉林农业大学中药材学院，吉林长春 130118吉林农业大学中药材学院，吉林长春 130118吉林农业大学中药材学院，吉林长春 130118
畜牧业
水貂阿留申病毒分离鉴定免疫过氧化物酶单层细胞试验
ADVisolationidentificationIPMA
《中国动物检疫》 2015 (12)
73-77,5
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