中国康复理论与实践Issue(12)：1385-1390,6.DOI:10.3969/j.issn.1006-9771.2015.12.005
AMPA受体参与大鼠胸髓前角发育早期突触传递的生理学特点
Physiological Characteristics of Synaptic Transmission of Anterior Horn Early Development in Thoracic Spinal Cord Mediated by AMPA Receptors in Rats
摘要
Abstract
Objective To explore the physiological characteristics of synaptic transmission of anterior horn early development in thorac-ic spinal cord mediated byα-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors in rats. Methods 36 Wistar rats were divided into embryonic 17 days group (E17, n=12), embryonic 20 days group (E20, n=12) and postnatal 7 days group (P7, n=12). Immuno-fluorescent staining of calmodulin-dependent protein kinaseⅡ(CaMKⅡ) was used to test the distribution of AMPA receptors. Multi-elec-trode array technique (MED-64 system) was used to test the changes of field excitatory post-synaptic potential (fEPSP) of synaptic transmis-sion mediated by AMPA receptor. Results There was small amount of CaMKⅡ-positive neurons existing in gray matter of spinal cord at E17, CaMKⅡ-positive neurons migrated to the center, and the number of neurons became more and more in E20 and P7 rats. The number of evoked fEPSP gradually increased in rats from E17 to P7, and could be blocked by 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX). The range of synaptic connection in spinal cord gray matter significantly reduced (P<0.001). Conclusion AMPA receptors participate in the early development of spinal cord in rats and act as the main excitatory receptor of functional synaptic connection in neural network of ventricornu.关键词
α-氨基-3-羟基-5-甲基-4-异恶唑丙酸受体/脊髓发育/场兴奋性突触后电位/突触联系/大鼠Key words
α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor/spinal cord development/field excitatory postsynaptic potential/synaptic connection/rats分类
医药卫生引用本文复制引用
张雯秀,段红梅,谢雅彬,李曼丽,杨朝阳,李晓光..AMPA受体参与大鼠胸髓前角发育早期突触传递的生理学特点[J].中国康复理论与实践,2015,(12):1385-1390,6.基金项目
1.国家“863”计划项目(No.2012AA020206)；2.国家自然科学基金面上项目(No.31271037)；3.国家自然科学基金国际合作与交流项目(No.31320103903)；4.“十二五”国家科技支撑计划项目(No.2012BAI17B04)；5.高等学校全国优秀博士学位论文作者专项资金资助项目(No.201356)；6.国家国际科技合作专项项目(No.2014DFA30640)；7.国家自然科学基金委员会资助项目(No.31130022)。 （No.2012AA020206）
