中国临床药理学杂志2016，Vol.32Issue(1)：39-41,3.DOI:10.13699/j.cnki.1001-6821.2016.01.013
Rho激酶在醛固酮诱导的血管平滑肌细胞增殖中的作用
Role of Rho kinase in vascular smooth muscle cell proliferation induced by aldosterone
摘要
Abstract
Objective To explore the role of Rho kinase in vascular smooth muscle cell ( VSMC) proliferation induced by aldosterone ( ALD) and the effect of mineralcocorticoid receptor (MR).Methods The ex-pressions of Rho -kinase protein were detected by Western blot .VSMC proliferation was measured by [ 3 H] Thymidine incorporation.Results ALD could up-regulate the expressions of Rho -kinase protein in dose-and time-dependent manner , peak at 20 min and 100 nmol · L-1 . Eplerenone , mineralcocorticoid receptor antagonist , could significantly reverse these effects , indicating the activation of Rho -kinase pathway induced by ALD in VSMC was mediated by MR .ALD could significantly induce VSMC proliferation , which could be inhibited by eplerenone and Y-276329 ( Rho-kinase inhibitor ) , indicating the VSMC proliferation induced by ALD was mediated by MR and the activation of Rho -kinase pathway.Conclusion Rho-kinase pathway and MR play an important role in VSMC proliferation induced by ALD .关键词
醛固酮/Rho激酶/醛固酮受体/血管平滑肌细胞/细胞增殖Key words
aldosterone/Rho -kinase/mineralcocorticoid receptor/vas-cular smooth muscle cell/cell proliferation分类
医药卫生引用本文复制引用
马春阳,万小平,杨堃,王子珍,郑传宜,叶富跃,吴然..Rho激酶在醛固酮诱导的血管平滑肌细胞增殖中的作用[J].中国临床药理学杂志,2016,32(1):39-41,3.基金项目
2014年海南省社会发展科技专项基金资助项目(SF201407) （SF201407）
2015年海南省中药现代化专项基金资助项目(2015ZY23) （2015ZY23）
海南省卫生厅科研立项基金资助项目(琼卫2009-44,琼卫2006-56) （琼卫2009-44,琼卫2006-56）
