中国药理学通报Issue(12)：1688-1692,5.DOI:10.3969/j.issn.1001-1978.2015.12.012
氧化苦参碱对高脂诱导胰岛素抵抗ApoE －／－小鼠肝脏脂代谢相关基因的影响
Effect of oxymatrine on lipid metabolism regulated genes in liver of fat-induced insulin resistance in ApoE -/-mice
摘要
Abstract
Aim To investigate the effect of oxymatrine on lipid metabolism regulated genes in liver in fat-in-duced insulin resistance in ApoE -/- mice.Methods Seventeen C57BL/6J male mice were selected in normal control group.Sixty-eight ApoE -/- mice with high fat diet for 1 6 weeks,were randomly divided into model group,oxymatrine low,middle and high dose groups.Then they were gavaged for 8 weeks.Body weight and general biochemical indicators were deter-mined in mice.The mRNA and protein expression lev-els of LPL,FAT/CD36,CPT1 ,UCP2,SREBP-1 c,FAS and ACC were examined by real-time PCR and West-ern blot in the liver.Results Compared with model group,oxymatrine reduced body weight(BW),fasting <br> blood glucose (FBG),cholesterol (TC ),triglyceride (TG),free fatty acids(FFA),fasting plasma insulin (FINS)and insulin resistance index(HOMA-IR)(P <0.05),while improved glucose infusion rate (GIR). Oxymatrine down-regulated the mRNA and protein ex-pression of LPL,FAT/CD36,UCP2,SREBP-1 c,FAS and ACC(P <0.05),and up-regulated the mRNA and protein expression of CPT1 in varying degrees (P <0.05).Conclusion Oxymatrine can regulate the ex-pression of lipid metabolism regulated genes in liver and improve insulin resistance in ApoE -/- mice in-duced by high fat diet.关键词
氧化苦参碱/高脂/ApoE -/-小鼠/胰岛素抵抗/脂代谢/肝脏Key words
oxymatrine/high fat diet/ApoE -/- mice/insulin resistance/lipid metabolism/liver分类
医药卫生引用本文复制引用
王杏,王超,宋光耀,费雯婕,刘小娜,张哲,马欢..氧化苦参碱对高脂诱导胰岛素抵抗ApoE －／－小鼠肝脏脂代谢相关基因的影响[J].中国药理学通报,2015,(12):1688-1692,5.基金项目
国家自然科学基金资助项目（No 81370900）；河北省重大医学科研项目 （）
