中国药理学通报Issue(12)：1725-1729,1730,6.DOI:10.3969/j.issn.1001-1978.2015.12.019
三叶青黄酮经 p38MAPK 和 NF-κB 途径抑制老年小鼠急性肺损伤
Inhibition effect of flavonoids from Radix tetrastigmae on acute lung injury of aged mice through p38MAPK and NF-κB pathway
摘要
Abstract
Aim To investigate the protective effect of flavonoids from Radix tetrastigmae (RTFs)on lipopo-lysaccharide (LPS)induced acute lung injury (ALI) of aged mice and the mechanism.Methods Aged C57BL/6J mice were bronchially instillated LPS to in-duce ALI.RTFs were orally administered to treat ALI. After 3 days,bronchoalveolar lavage fluid (BALF) was collected to enumerate leukocytes with Wright-Gi-emsa staining,and to detect inflammatory cytokines with ELISA.ELISA and Western blot methods were al-so used to detect the expression of MAPKs and NF-κB in lung tissues.The activity of NF-κB in nucleic pro-tein extract was detected with TransAMkit.Results <br> ALI models were successfully induced through LPS in-stillation.RTFs significantly reduced leukocyte,espe-cially neutrophil infiltration in BALF,inhibited IL-1 β, IL-6,IL-1 2p40,TNF-αand sTNF-R1 secretion,and improved pathohistological change of lung tissues.Be-sides,RTFs significantly attenuated the phosphoryla-tion of p38MAPK,NF-κB and the activity of NF-κB. Conclusion RTFs inhibits LPS-induced ALI through p38MAPK and NF-κB pathway and exhibits significant anti-inflammation effect on aged mice.关键词
三叶青/黄酮/p38MAPK/NF-κB/脂多糖/急性肺损伤/老年小鼠Key words
Radix tetrastigmae/flavonoids/p38MAPK/nuclear factor-κB (NF-κB)/lipopolysac-charide (LPS)/acute lung injury (ALI)/aged mice引用本文复制引用
刘丹丹,曹纲,张琦,叶夷露,韩立凯,葛卫红..三叶青黄酮经 p38MAPK 和 NF-κB 途径抑制老年小鼠急性肺损伤[J].中国药理学通报,2015,(12):1725-1729,1730,6.基金项目
浙江省中药现代化专项资助 （）
