中国药理学与毒理学杂志Issue(6)：960-966,7.DOI:10.3867/j.issn.1000-3002.2015.06.013
基于毒性整体早期评价的“十八反”中药配伍禁忌毒性表征的研究思路及方法
Research methods of ″18 incompatible medicaments″incompatibilitytoxicitycharacterizationoftraditional Chinese medicine based on early evaluation of overall toxicity
摘要
Abstract
Currently,the toxicity study of traditional Chinese medicine is faced with the following problems. Firstly,the evaluation in vitro cannot fully reflect the true state of the body. Secondly,the traditional method is not sensitive enough to the early toxicity. Lastly,the toxicity evaluation indexes cannot determine whether the compatibility of traditional Chinese medicine produces toxicity or increases toxicity systematically. The paper proposed a synthesized early evaluation research method for target organ toxicity induced by traditional Chinese medicine:screening,validation,optimization and application. This method mainly inoolves early target organ toxicity biomarkers in screening,optimi⁃zation,validation,biological significance explanation,and application to the traditional Chinese medicine incompatibility based on the metabolic dynamic fingerprint spectrum in order to obtain biomarkers of target organ toxicity that are sensitive and precede conventional biochemical indices for early evaluation . We attempted to analyze the pattern of chang of the biomarkers for animals acted by″18 incompatible medicaments″compatibility combination. We found that Radix Aconiti Lateralis Preparata with cardiotoxicity were compatible with Rhizoma Pinelliae,and that Trichosanthes kirilowii Maxim,Fritillaria,Ampelopsis Radix and Bletilla striata without non-cardiotoxicity produced and increased cardiotoxicity systematically.关键词
十八反/中草药/药物配伍禁忌/早期毒性/代谢组学/生物标志物Key words
18 incompatible medicaments/drugs,Chinese herbal/drug incompatibility/early toxicity/metabolomics/biomarkers分类
医药卫生引用本文复制引用
李遇伯,局亮,邓皓月,张振珠,王磊,侯治国,尹佳,张艳军..基于毒性整体早期评价的“十八反”中药配伍禁忌毒性表征的研究思路及方法[J].中国药理学与毒理学杂志,2015,(6):960-966,7.基金项目
国家重点基础研究发展计划（2011CB505300）；国家重点基础研究发展计划（2011CB505302）；国家自然科学基金项目（81273998）；国家自然科学基金项目（81573825）；中国博士后特别资助项目（133806）@@@@The project supported by the National Basic Research Program of China （2011CB505300）；National Basic Research Program of China 2011CB505302）；National Natural Science Foundation of China （81273998）；National Natural Science Foundation of China（81573825）；and China Postdoctoral Science Foundation Funded Project （）
