高效液相色谱－二极管阵列检测器法测定保健食品和中成药中违法添加的10种降糖类西药OA
Detection of 10 Kinds of Antidiabetics Illegally Added in Traditional Chinese Medicines and Health Care Products by HPLC-DAD
目的：建立高效液相色谱－二极管阵列检测器（HPLC－DAD）法，测定保健食品和中成药中添加的10种降糖类西药。方法色谱柱采用Agilent ZORBAX SB－C18柱（250 mm ×4.6 mm，5μm），流动相为0.01 mol/L的乙酸铵水溶液（A）－甲醇（B）梯度洗脱，进样量为10μL，DAD扫描范围为190~400 nm，检测波长为235 nm，流速为1.0 mL/min。通过比较样品峰与对照品峰的保留时间，DAD光谱、一级质谱、二级…查看全部>>
Objective To establish an HPLC-DAD method for the determination of 10 antidiabetic agents illegally added in traditional Chinese medicines and health care products. Methods The analytes were separated on an Agilent ZORBAX SB-C18 column(250 mm × 4. 6 mm,5 μm)with 0. 01 mol/L ammonium acetate solution and methanol as mobile phase by gradient elution. DAD scanning range was 190-400 nm,detection wavelength of 235 nm,flow rate of 1. 0 mL/min. The retention time,D…查看全部>>
隋海山;王立娟;谢菲
山东省潍坊市食品药品检验检测中心，山东 潍坊 261041山东省潍坊市红十字中心血站，山东 潍坊 261041山东省潍坊市食品药品检验检测中心，山东 潍坊 261041
医药卫生
保健品非法添加降糖药高效液相色谱-二极管阵列检测器
healthy care productsadded illegallyantidiabeticsHPLC-DAD
《中国药业》 2015 (22)
110-111,112,3
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