中国药业Issue(22)：114-115,2.
高效液相色谱法测定半夏糖浆中麻黄碱和伪麻黄碱含量
Determination of Pseudoephedrine in Pinellia Syrup by HPLC
付静 1朱月月 1聂诗明1
作者信息
- 1. 湖北中医药大学药学院，湖北 武汉 430065
- 折叠
摘要
Abstract
Objective To establish a method for determining ephedrine and pseudoephedrine in pinellia syrup by HPLC. Methods Welch Ultimate XB-C18(250 mm × 4. 6 mm,5 μm)column was used. 0. 02 mo/L sodium dihydrogen phosphate was used as buffer (0. 4% Triethylamine,pH=3. 0)- methanol(10 : 90) was used as mobile phase. The flow rate was 1 mL/min. The detection wave-length was at 210 nm. Results The liner range of ephderine and pseudoephedrine were 1. 934 4-92. 85 μg/mL and 2. 481 3-119. 10 μg/mL. The equations of linear regression were Y=0. 888 8 X-0. 067 2,r=1. 000 0 ( n=6 ) and Y=0. 691 1 X-0. 139 6, r=1. 000 0 ( n=6 ) . The average recovery of ephedrine and pseudoephedrine were all > 95. 00% ( n=6 ) . Conclusion This method is simple,can separate the contents completely,and can be used as content determination method of ephedrine and pseudoephedrine in pinellia syrup.关键词
半夏糖浆/麻黄碱/伪麻黄碱/高效液相色谱法Key words
pinellia syrup/ephderine/pseudoephderine/HPLC分类
医药卫生引用本文复制引用
付静,朱月月,聂诗明..高效液相色谱法测定半夏糖浆中麻黄碱和伪麻黄碱含量[J].中国药业,2015,(22):114-115,2.
