中国药业Issue(22)：121-121,122,2.
反相高效液相色谱法测定盐酸倍他司汀氯化钠注射液中主药含量
Content Determination of Betahistine Hydrochloride in Betahistine Hydrochloride Sodium Chloride Injection by RP-HPLC
逯小萌1
作者信息
- 1. 河南省濮阳市食品药品检验所，河南 濮阳 457000
- 折叠
摘要
Abstract
Objective To establish an RP-HPLC method for the Content Determination of betahistine hydrochloride in Betahistine Hy-drochloride Sodium Chloride Injection. Methods The chromatographic column was Thermo-C18 column(250 mm × 4. 6 mm,5 μm);the mobile phase was 0. 01 mol/L sodium acetate buffer(containing 0. 004 mol/L heptane sulfonic acid sodium salt,0. 2% triethylamine,regu-lated to pH 4. 0 glacial acetic acid)-methanol(70 :30);the flow rate was 1. 0 mL/min;the column temperature was 30 ℃;the detection wavelength was 261 nm. Results Betahistine hydrochloride showed good linear relationship with the peak area in the range of 0. 102 3-1. 023 00 μg( r=0. 999 9),the average recovery was 99. 61%,RSD=0. 21%( n=9). Conclusion This method is accurate,sensitive and repeatable,and can be used as the content determination of betahistine hydrochloride in Betahistine Hydrochloride Sodium Chloride Injection.关键词
盐酸倍他司汀氯化钠注射液/盐酸倍他司汀/反相高效液相色谱法/含量测定Key words
Betahistine Hydrochloride Sodium Chloride Injection/betahistine hydrochloride/RP-HPLC/content determination分类
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逯小萌..反相高效液相色谱法测定盐酸倍他司汀氯化钠注射液中主药含量[J].中国药业,2015,(22):121-121,122,2.
