中国中医药信息杂志Issue(1)：96-99,4.DOI:10.3969/j.issn.1005-5304.2016.01.023
顽痹清丸HPLC指纹图谱研究
Study on HPLC Fingerprint ofWanbiqing Pills
罗石任 1王昭 1李洁1
作者信息
- 1. 河南省洛阳正骨医院河南省骨科医院，河南郑州 450000
- 折叠
摘要
Abstract
Objective To establish an evaluation system of HPLC fingerprint of Wanbiqing Pills; To provide references for the quality control ofWanbiqing Pills.Methods Wanbiqing Pills were analyzed with HPLC method. Analysis was performed on Agilent C18 column (4.6 mm×250 mm, 5μm) with the mobile phase of acetonitrile- 0.1%H3PO4 water gradient elution at the flow rate of 1.0 mL/min. The detection wavelength was 258 nm. Sample injection was 20μL. Standard fingerprint ofWanbiqing Pills was established based on Similarity Evaluation System for Chromatographic Fingerprint of TCM (Version 2004 A). The similarity ofWanbiqing Pills from different batches was computed.Results A standard HPLC fingerprint procedure was developed forWanbiqing Pills with 20 common peaks. The similarity of 10 batches ofWanbiqing Pills was not lower than 0.98. Five peaks received chemical confirmation.Conclusion The HPLC method is stable and feasible and with good repeatability, which can be used for the quality control ofWanbiqing Pills.关键词
顽痹清丸/高效液相色谱法/指纹图谱Key words
Wanbiqing Pills/HPLC/fingerprint分类
医药卫生引用本文复制引用
罗石任,王昭,李洁..顽痹清丸HPLC指纹图谱研究[J].中国中医药信息杂志,2016,(1):96-99,4.
