中国中医药信息杂志Issue(1)：103-105,3.DOI:10.3969/j.issn.1005-5304.2016.01.025
HPLC同时测定痛经灵颗粒中3种有效成分含量
Simultaneous Determination of Three Active Ingredients inTongjingling Granules by HPLC
李中娥1
作者信息
- 1. 南阳市食品药品检验所，河南南阳 473061
- 折叠
摘要
Abstract
Objective To establish a simultaneous determination method for Salvianolic acid B, Paeoniflorin and Hydroxysafflor yellow A inTongjingling Granules; To provide a reliable method for effective quality control. Methods The determination was performed on a Welchrom C18 column with acetonitrile (A) - 0.1% phosphoric acid (B) as the mobile phase in gradient elution mode at the flow rate of 1.0 mL/min. The detection wavelength was 230 nm. Results Salvianolic acid B presented in a good linear relation in the range of 0.197–1.316μg, and the average recovery rate was 98.72% with RSD 1.49%; Paeoniflorin presented in a good linear relation in the range of 0.158–1.051μg, and the average recovery rate was 99.01% with RSD 0.92%; Hydroxysafflor yellow A presented in a good linear relation in the concentration range of 0.053–0.353μg, and the average recovery rate was 98.34% with RSD of 1.60%.Conclusion This method is simple, sensitive, accurate and with good reproducibility, which can be used for quality control ofTongjingling Granules.关键词
痛经灵颗粒/丹酚酸B/芍药苷/羟基红花黄色素A/高效液相色谱法Key words
Tongjingling Granules/Salvianolic acid B/Paeoniflorin/Hydroxysafflor yellow A/HPLC分类
医药卫生引用本文复制引用
李中娥..HPLC同时测定痛经灵颗粒中3种有效成分含量[J].中国中医药信息杂志,2016,(1):103-105,3.
