北华大学学报（自然科学版）2016，Vol.17Issue(1)：43-46,4.DOI:10.11713/j.issn.1009-4822.2016.01.010
复方北五味子促睡眠口服液镇静催眠作用及机制研究
Sedative and Hypnotic Effects and Mechanisms of Co Schisandra Sleeping-Promoting Oral Liquid
摘要
Abstract
Objective To observe the sedative and hypnotic effects of Co Schisandra sleep-promoting oral liquid ( CSSPOL) and to explore the underlying mechanisms. Method A total of 40 male ICR mice were randomly divided into 4 groups with 10 per group,namely,normal control (distilled water),low-dose (4 times diluent), moderate-dose ( 2 times diluent ) and high-dose ( stock solution ) Co Schisandra sleep-promoting oral liquid groups. The mice were orally administrated at 0. 1 mL/10 g,twice per day for 7 consecutive days. After the last administration,the independent activity recorder was used to observe the effect of CSSPOL on autonomic activities of the mice. The hypnotic effect of CSSPOL on mice treated with sodium pentobarbital was observed. The contents of γ-aminobutyric acid ( GABA) ,glutamate ( Glu) and glutamine ( Gln) in the brains of mice were detected with ELISA method for exploring the underlying mechanisms. Results Compared with the control group, CSSPOL could reduce the total number of autonomic activities in mice,shorten the sleep latency and increase the number of sleeping mice treated with sodium pentobarbital ( sub-threshold dose) . Furthermore,CSSPOL could prolong the sleeping time of the mice treated with sodium pentobarbital (threshold dose). CSSPOL could also significantly decrease the contents of Glu and Gln in mice brain tissues. Conclusion CSSPOL has the sedative and hypnotic effects in mice,which might be related to the reduction of inhibitory neurotransmitter Glu and Gln in mice brain.关键词
复方北五味子促睡眠口服液/镇静/催眠Key words
Co Schisandra sleep-promoting oral liquid/sedation/hypnosis分类
医药卫生引用本文复制引用
王春梅,孙卉,刘歌明,李贺,孙靖辉,陈建光..复方北五味子促睡眠口服液镇静催眠作用及机制研究[J].北华大学学报（自然科学版）,2016,17(1):43-46,4.基金项目
吉林省科技厅医药产业发展专项基金( YYZX201124-1) . （ YYZX201124-1）
