北华大学学报（自然科学版）2016，Vol.17Issue(1)：58-61,4.DOI:10.11713/j.issn.1009-4822.2016.01.014
温阳抗寒合剂治疗支气管哮喘小鼠作用机制实验研究
Experimental Study on Effect Mechanism of Wen Yang Kan Han Mixture on Bronchial Asthma in Mice
摘要
Abstract
Objective To observe the therapeutic effect and underlying mechanism of traditional Chinese preparation Wen Yang Kang Han Mixture (WYKHM)on bronchial asthma in mice. Method Sixty Balb/c mice were randomized into six groups,asthmatic group,aminophylline group,control group,high dose group (200 mg/kg),middle dose group (100 mg/kg) and low dose group (50 mg/kg). The mice were sensitized by injecting ovalbumin except the control group and the experimental groups were administrated WYKHM after 1 week. After 2 weeks,ovalbumin was given to the mice to induce asthma. Then,the blood was taken from orbital vein in all the mice after 48 hours. D-Hanks balanced liquor was lavaged into bronchial alveolus to draw BALF. The concentrations of IL-5,IL-23,NO, NOS,SOD and MDA in BALF were measured. Results Compared with the asthmatic group,WYKHM decreased the contents of NO and NOS in BALF dramatically (P<0. 05 or P<0. 001). IL-5 concentrations of WYKHM groups and aminophylline group were lower than that of the asthmatic group with obvious difference ( P<0. 05 or P<0. 001). Compared with the control group and asthmatic group,SOD levels and MDA levels of the treatment groups were significantly higher and lower,respectively (P<0. 05 or P<0. 01). Conclusion WYKHM could alleviate asthmatic symptoms by decreasing NO level, MDA level and NOS activity, by elevating SOD content, and by regulating the key factor of IL-5 and IL-23.关键词
支气管哮喘/白介素-5/白介素-23/温阳抗寒合剂Key words
bronchial asthma/interleukin-5/interleukin-23/Wen Yang Kan Han mixture分类
医药卫生引用本文复制引用
李昕辉..温阳抗寒合剂治疗支气管哮喘小鼠作用机制实验研究[J].北华大学学报（自然科学版）,2016,17(1):58-61,4.基金项目
吉林市科技局科技发展计划项目 (200903010). （200903010）
