北华大学学报（自然科学版）2016，Vol.17Issue(1)：66-68,3.DOI:10.11713/j.issn.1009-4822.2016.01.016
溶血对氧化酶法测定葡萄糖含量影响的实验研究
Experimental Study on Effect of Hemolysis on Glucose Content Determined by Oxidase Method
摘要
Abstract
Objective To investigate the effect of hemolysis on the determination of glucose in blood sample,and to find method for correction. Method Total 900 cases from normal healthy blood samples were randomly selected in this study. The hemolysis standard was evaluated according to the concentration of plasma free hemoglobin,and the concentration of glucose was tested by dual wavelength spectrophotometry. A regression based on two element equation was used to determine the corrected values. Results When hemolysis appeared, the measured value of glucose concentration was lower than the actual value. And the more serious is the hemolysis the more obvious became the difference. The final regression equation was Z=0. 415+0. 053X+0. 014Y,with correlation coefficient r=0. 974. After regression,the uncorrected deviation value (δ1 ) and corrected deviation value (δ2)were -13. 4% and -7. 7%,respectively,and the difference was significant (P<0. 05). The value ofδ2 was under allowing ( ± 0 . 1 ) . Conclusion The difference between corrected measured values and actual values of glucose concentration is within the range after correcting regression equation.关键词
标本溶血/氧化酶法测定葡萄糖/影响/纠正Key words
specimen hemolysis/glucose oxidase method/influence/correction分类
医药卫生引用本文复制引用
包学英,王丹峰..溶血对氧化酶法测定葡萄糖含量影响的实验研究[J].北华大学学报（自然科学版）,2016,17(1):66-68,3.基金项目
吉林省教育厅科学技术研究项目(20120230). （20120230）
