湖北民族学院学报(自然科学版)Issue(4):421-424,4.DOI:10.13501/j.cnki.42-1569/n.2015.12.017
小白菜与大白菜亚种间杂交减数分裂染色体行为观察
Chromosome Behaviors in Meiotic of Pollen Mother Cells in Inter-subspecific Hybridization between Chinese Cabbage and Non-heading Chinese Cabbage
摘要
Abstract
Meiotic behaviors of pollen mother cells ( PMCs) in inter-subspecific hybrid F1 and BC1-3 be⁃tween Chinese cabbage and non-heading Chinese cabbage were studied. It was observed that 90.7%of F1 pollen were stainable, indicating good fertility. The fertility of BC1-3 was gradually increased. 89.1%chro⁃mosome behavior in F1 meiotic PMCs was regular which could explain the good fertility. Abnormal chro⁃mosome behaviors such as double nucleoli, disordered paring in Prophase I, chromosome bridge in meta⁃phase I, segments loss in anaphase I were discovered which could explain the lower fertility of F1 hybrid than normal diploid. 14.3% abnormal chromosome behaviors were found in BC1 generation however very limited in BC2-3 which indicated that it was a repaid process for chromosome behavior of backcross genera⁃tion becoming normal;this result was consistent to phenotype and fertility investigation.关键词
白菜/亚种间杂交/减数分裂/染色体行为Key words
Chinese cabbage/inter-subspecific hybrid/meiosis/chromosome behaviors分类
农业科技引用本文复制引用
陈龙正,徐海,宋波,樊小雪..小白菜与大白菜亚种间杂交减数分裂染色体行为观察[J].湖北民族学院学报(自然科学版),2015,(4):421-424,4.基金项目
江苏省自然科学基金项目(BK20130715);农业部公益性行业(农业)科研专项经费项目(201403032). ()