海洋科学2015，Vol.39Issue(11)：7-12,6.DOI:10.11759/hykx20150612
皱纹盘鲍群体选育第四代成鲍4种多糖裂解酶基因表达的初步研究
Expression of polysaccharidase enzymes in the F4 adults of a mass selected Pacific abalone strain
摘要
Abstract
Alginatelyase, laminarinase, α-amylase and cellulase gene segments were amplified in DNA and cDNA templates from the 2-year-old F4 adults of '97 selected Pacific abalone strain with primers designed based on pub-lished cDNA sequences of these genes. Sequencing of these gene segments revealed one intron of 438 bp in theα-amylase gene, one intron of 667 bp in the cellulase gene, and two SNPS in both exon and intron of the cellulase. Semi-quantitative RT-PCR and Real-time PCR analyses showed that the four enzymes were mainly expressed in the digestive gland, with the alginatelyase gene expression the highest, followed by the cellulose gene, suggesting that alginate may be one of the most important carbohydrate sources in the selected abalone strain fed with kelp. The results in this study may form the basis for further studies on the expression of polysaccharidases of the '97 strain at different developmental stages, fed with different macroalgae and under different aquatic environments.关键词
皱纹盘鲍(HaliotisdiscushannaiIno)/褐藻酸酶/淀粉酶/纤维素酶/基因表达Key words
Haliotis discus hannai Ino/alginate lyase/amylase/cellulase/mRNA expression分类
农业科技引用本文复制引用
何庆国,王劭雯,李加琦,刘晓..皱纹盘鲍群体选育第四代成鲍4种多糖裂解酶基因表达的初步研究[J].海洋科学,2015,39(11):7-12,6.基金项目
国家863计划项目(2012AA10A412) （2012AA10A412）
