海洋科学2015，Vol.39Issue(11)：49-53,5.DOI:10.11693/hyhz20150731005
紫贻贝贝壳和脉红螺厣中色素的提取与鉴定
Extraction and identification of the pigment in the Mytilus edulis shells and Rapana venosa operculum
摘要
Abstract
To determine if the black substance in the Mytilus edulis shells and Rapana venosa operculum is melanin, we selected the Rapana venosa operculum part and collected M. edulis shells, cleaned the two samples and then pulverized them, weighed, followed by hydrochloric acid soaking, boiling water bath for heating, as well as diethyl ether purification step, then, the dissolved black substance in 0.01 mL/L NaOH, the maximum absorption of the two pigments in the UV absorption spectrum within the range of 190–500 nm was both observed between 210–220 nm. The UV absorbance decreased with increasing wavelength which was consistent with the UV spectral absorption characteristics of melanin. In addition, Fourier transform infrared spectroscopy scanning revealed characteristic absorption peaks that emerged near 3 μm and 6 μm, which was consistent with infrared scanning features of eume-lanin. Thus the black substance in the Rapana venosa operculum and M. edulis shells was confirmed to be melanin for the first time.关键词
紫贻贝(Mytilusedulis)贝壳/脉红螺(Rapanavenosa)厣/黑色素鉴定/紫外吸收光谱/傅里叶红外扫描图谱Key words
Mytilus edulis shell/Rapana venosa operculum/melanin identification/ultraviolet spectrum/infrared radiation spectrum分类
海洋科学引用本文复制引用
郝世鑫,侯鑫,孙东方,于文超,何成,王晓通..紫贻贝贝壳和脉红螺厣中色素的提取与鉴定[J].海洋科学,2015,39(11):49-53,5.基金项目
国家自然科学基金资助项目(31302181) （31302181）
山东省重点研发计划(2015GSF115013) （2015GSF115013）
山东省自然科学基金资助项目(ZR2013CM026) （ZR2013CM026）
烟台市科技发展计划资助项目(2013ZZ357) （2013ZZ357）
