吉林中医药Issue(1)：65-70,71,7.DOI:10.13463/j.cnki.jlzyy.2016.01.018
清金化痰汤对 COPD 模型大鼠肺组织中性粒细胞弹性蛋白酶及黏蛋白5AC 表达的影响
Qingjinhuatan decoction adjusting chronic obstructive pulmonary model rat in airway mucus hypersecretion by neutrophil elastase and mucin 5AC
摘要
Abstract
Objective To explore Qingjinhuatan decoction’s mechanism on airway mucus hypersecretion of chro-nic obstructive pulmonary disease rat model. Methods Clean level Wistar rats(n = 40)are randomly divided into blank control group(A),model group(B),Qingjinhuatan decoction group(C)and Clarithromycin group(D). Using dripping LPS in bronchus and smoking to intervene on B,C and D group rat in order to build COPD airway mucus hy-persecretion model. B,C and D group rats are separately raised by normal saline,Qingjinhuatan decoction and Clar-ithromycin for 30 days,at the same time blank control group rat are raised normally. All rats are killed on 31th day, and using RT-PCR method to observe HEmRHA,MUC 5ACmRHA expression on lung tissue,Alcian blue periodic acid Schiff staining method to observe hyperplasia of goblet cells on airway epithelium,Immunohistochemical method to test HE and MUC 5AC expression on pulmonary tissue and airway epithelium. Results Model group rats’HEmRHA,MUC 5ACmRHA expression,goblet cells’hyperplasia on airway epithelium,HE and MUC 5AC expression on pulmonary tis-sue and airway epithelium are significant increase than blank control group;Qingjinhuatan decoction and Clarithromy-cin group rats’MUC 5ACmRHA,goblet cells’hyperplasia on airway epithelium,MUC 5AC expression on pulmonary tissue and airway epithelium is significant less than model group;Qingjinhuatan decoction group rats’HEmRAH,MUC 5ACmRHA,HE expression on pulmonary tissue and airway epithelium is significant less than Clarithromycin group;Qingjinhuatan decoction group rats’goblet cells’hyperplasia on airway epithelium and MUC 5AC expression on pulmo-nary tissue and airway epithelium have on difference with Clarithromycin group. Conclusion Qingjinhuatan decoction can reduce airway mucus hepersecrection of chronic obstructive pulmonary disease by HE / MUC 5AC pathway.关键词
清金化痰汤/中性粒细胞弹性蛋白酶/慢性阻塞性肺疾病/气道黏液高分泌/黏蛋白 5ACKey words
Qingjinhuatan decoction/neutrophil elastase/chronic obstructive pulmonary/airway mucus hyperse-cretion/Mucin 5AC分类
医药卫生引用本文复制引用
陈英,冯淬灵,李根茂,葛东宇,王骏,李春盈,姚小芹..清金化痰汤对 COPD 模型大鼠肺组织中性粒细胞弹性蛋白酶及黏蛋白5AC 表达的影响[J].吉林中医药,2016,(1):65-70,71,7.基金项目
北京市自然基金资助项目（7132115）。 （）
