江西农业大学学报Issue(6)：1094-1099,6.DOI:10.13836/j.jjau.2015167
金针菇菌糠中木聚糖酶的分离纯化及其酶学性质研究
Purification and Properties of Xylanase from Spent Mushroom Compost of Flammulina velutipes
摘要
Abstract
Xylanase produced by Spent Mushroom Compost was isolated and purified by ammonium sulfate salting-out,Sephadex G⁃100 column chromatography and DEAE C⁃52 anion chromatography exchange,and its enzymatic properties were studied.The results illustrated that it exhibited a single band by the SDS⁃PAGE,and the molecular weight was estimated as 37. 8 KDa. The purification made the specific activity as high as 946.67 U/mg and purification multiple reached 12.74.The optimal temperature and pH value for activity were 50 ℃ and pH 6.0 respectively,which had a certain acid and alkali resistance and high⁃temperature⁃resisting.Xy⁃lanase obtained in this study was activated by Fe2+and Zn2+;promoted by Mn2+,K+and Ca2+;while inhibited by Mg2+,Cu2+ and Fe3+.The Vmax and Km values of the xylanase for xylan were 15.43μg/mL/min and 9.61 mg/mL, respectively.The study suggests that this xylanase has a good potential to be a cold⁃stable xylanase in paper man⁃ufacturing and feed industries.关键词
木聚糖酶/分离纯化/酶学性质Key words
xylanase/purification/enzymatic properties分类
农业科技引用本文复制引用
杨云龙,黄彩梅,白植成,胡开辉..金针菇菌糠中木聚糖酶的分离纯化及其酶学性质研究[J].江西农业大学学报,2015,(6):1094-1099,6.基金项目
国家自然科学青年基金项目（21407024）和福建省教育厅项目 （）
