江西农业学报Issue(12)：1-6,6.
农杆菌介导的籼型水稻恢复系 R752成熟胚转基因技术体系的优化
Optimization of Agrobacterium-mediated Transgenic Technology System for Indica Rice Restorer R752 by Using Mature Embryo
摘要
Abstract
In this study , the Agrobacterium-mediated transgenic technology system for indica late rice restorer R 752 by using mature embryo callus as recipient was optimized .The results showed that the MSS medium was better for callus induction from ma-ture embryo than the CCS medium .It was also found that maltose used as carbon source was better for callus induction than sucrose at the same concentration .The combination of callus induction medium MSM and differentiation medium MSB obtained the highest green plantlet production rate (28.97%).The results of orthogonal experiment showed that the optimal concentration of 6-BA, NAA and KT in differentiation medium was 2.0 mg/L, 0.5 mg/L and 1.0 mg/L, respectively, and NAA had the greatest effect on green plantlet production rate , followed by KT and 6-BA in order.The infection efficiency of three tested Agrobacterium strains for primary calli induced from mature embryo revealed the following order: EHA105>LBA4404>GV3101.Using EHA105, the Bt gene was transformed into the mature embryo callus of R 752 by the constructed high-efficient Agrobacterium-mediated transgenic technology system, and the average transformation efficiency was 10.98%.关键词
籼稻/R752/农杆菌介导/转基因/成熟胚/体系优化Key words
Indica rice/R752/Agrobacterium-mediated transformation/Transgene/Mature embryo/Optimization分类
农业科技引用本文复制引用
黄仁良,沈显华,熊宏亮,HninPwint Wai,朱珊,严松,邓伟,沈林军,魏永清,陈丽妹..农杆菌介导的籼型水稻恢复系 R752成熟胚转基因技术体系的优化[J].江西农业学报,2015,(12):1-6,6.基金项目
国家转基因生物新品种培育重大专项（2014ZX08001－001）；江西省主要学科学术和技术带头人培养计划项目（060006）。 （）
