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应用 CantoⅡ流式细胞仪建立14~16岁人群 CD64正常参考值的探讨∗

吕颖 闫丹丹 张巍 刘玉珍 卢建华 段媛媛 戴二黑 闫会敏

检验医学与临床Issue(1):29-30,2.
检验医学与临床Issue(1):29-30,2.DOI:10.3969/j.issn.1672-9455.2016.01.012

应用 CantoⅡ流式细胞仪建立14~16岁人群 CD64正常参考值的探讨∗

Establishment of normal reference value for CD64 in healthy adolescents aged 14 - 1 6 years by Canto Ⅱ flow ;cytometer

吕颖 1闫丹丹 2张巍 3刘玉珍 1卢建华 1段媛媛 1戴二黑 4闫会敏1

作者信息

  • 1. 河北省石家庄市第五医院肝病研究所 050021
  • 2. 河北医科大学研究生学院,石家庄 050017
  • 3. 河北师范大学生命科学学院,石家庄 050024
  • 4. 河北医科大学研究生学院,石家庄 050017
  • 折叠

摘要

Abstract

Objective To investigate the normal reference value establishment of CD64 in the healthy adoles-cents aged 14-1 6 years by the CantoⅡ flow cytometer.Methods The peripheral blood samples collected from 52 healthy adolescents aged 14-1 6 years were detected the CD64 expression by using the BD CantoⅡ flow cytometer, and the results were statistically analyzed.Results The expression of CD64 was highest in monocytes,but almost no expression was found in lymphocytes.The CD64 mean fluorescence intensity (MFI)in neutrophils was 782.78,its 95% normal reference range was 21 1.78 - 1 353.78,the 95% normal reference range of CD64 index was less than 1.53.Conclusion The method for detecting the normal reference value of CD64 in the healthy adolescents by the CantoⅡ flow cytometer is preliminarily established,providing a reference for the basic researches and clinical diano-sis.

关键词

CantoⅡ流式细胞仪/CD64/正常参考值

Key words

CantoⅡ flow cytometer/CD64/normal reference value

引用本文复制引用

吕颖,闫丹丹,张巍,刘玉珍,卢建华,段媛媛,戴二黑,闫会敏..应用 CantoⅡ流式细胞仪建立14~16岁人群 CD64正常参考值的探讨∗[J].检验医学与临床,2016,(1):29-30,2.

基金项目

河北省医学科学研究课题计划项目(20130294) (20130294)

河北省中医药管理局基金资助项目(2013109)。 (2013109)

检验医学与临床

1672-9455

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