热带地理Issue(1)：108-113,6.DOI:10.13284/j.cnki.rddl.002801
南海北部陆坡表层沉积物氨氧化古菌多样性初探
Diversity of Ammonia-Oxidizing Archaea in the Surface Sediments of the Northern Continental Slope of the South China Sea
摘要
Abstract
Surface sediment samples were taken from three sites of different water depths at the northern continental slope of the South China Sea to investigate the community structure of ammonia-oxidizing archaea (AOA). Polymerase chain reaction (PCR) was employedto amplify the archaealammonia monooxygenase αsubunit(amoA) gene of AOA. Amplicons of theamoAgene sequences were used to understand the diversity and phylogenetic relationship of AOA. The main results were as follows: 1) All of thearchaealamoAgenesequences from the three locations were affiliated toGroup I.1aThaumarchaeota; 2)all ofthe archaealamoAgene sequences from the three locations were phylogenetically closely related; and 3) the16S rRNA gene andamoAgene phylogenetic trees were ofcongruent topology.Thaumarchaeota accounted for 40% of the total archaea, indicating that AOA might play an important role in the nitrogen biogeochemical cycling in the surface sediments of the northern continental slope of the South China Sea.关键词
氨氧化古菌/amoA基因/表层沉积物/南海北部Key words
Ammonia-Oxidizing Archaea/amoAgene/surface sediment/northern South China Sea分类
生物科学引用本文复制引用
刘国辉,吴后波..南海北部陆坡表层沉积物氨氧化古菌多样性初探[J].热带地理,2016,(1):108-113,6.基金项目
国家重大科学研究计划（2013CB956101）；国家自然科学基金项目（91428203）；中科院先导项目 （）
