肿瘤预防与治疗Issue(6)：317-321,334,6.DOI:10.3969/j.issn.1674-0904.2015.06.003
靶向抑制 EGFR 核转位对鼻咽癌细胞辐射耐受相关分子 DNA-PK 活化的作用
Inhibition of Targeting Nuclear Translocation of EGFR on Expression of DNA-PK Activation associated with Radioresistance in Nasopharyngeal Carcinoma Cells
摘要
Abstract
Objective:To investigate the effect of EGFR nuclear translocation inhibitory peptide on the expression of radioresistance related nuclear DNA-PK in nasopharyngeal carcinoma cells and its potential molec-ular mechanism.Methods:CNE-1 cells were pretreated with EGFR nuclear translocation inhibitory peptide (pT654)and control peptide (5 μM)for 16h,then the cells were exposed to 4Gy 60 Co irradiation.Western blot was used to determine the expression of EGFR,pEGFRThr654 ,DNA-PK and pDNA-PKThr2609 in cytoplasm and cell nucleus of CNE-1 cells at different time points,and the relationship between pDNA-PKThr2609 and pEG-FRThr654 was also investigated.Results:The expression of EGFR and pEGFRThr654 in CNE-1 cells increased sig-nificantly after the radiation,and the expression of pEGFRThr654 was positively correlated with pDNA-PKThr2609 (R2 =0.935).The inhibitory peptides of EGFR nuclear translocation (pT654)significantly decreased the ex-pression of nuclear pEGFRThr654 and pDNA-PKThr2609 (R2 =0.962).Conclusion:The inhibitory peptides of EG-FR nuclear translocation may inhibit the radiation-induced nuclear EGFR translocation and the DNA-PK activa-tion associated with the NHEJ repair pathway mediated by nuclear EGFR in nasopharyngeal carcinoma cells.关键词
人类表皮生长因子受体/非同源末端连接/DNA -依赖的蛋白激酶/鼻咽癌细胞Key words
EGFR/Non Homologous End Repair/DNA-PK/Nasopharyngeal Carcinoma分类
医药卫生引用本文复制引用
马士淇,李璐,刘惠芬,郎锦义,黄建鸣..靶向抑制 EGFR 核转位对鼻咽癌细胞辐射耐受相关分子 DNA-PK 活化的作用[J].肿瘤预防与治疗,2015,(6):317-321,334,6.基金项目
四川省卫生厅科研课题 （）
