上海针灸杂志Issue(1)：81-84,4.DOI:10.13460/j.issn.1005-0957.2016.01.0081
电针对糖尿病性胃轻瘫模型大鼠INS水平和CCK含量的影响
Effect of Electroacupuncture on the INS Level and CCK Content in a Rat Model of DGP
摘要
Abstract
Objective To investigate the effect of electroacupuncture on serum insulin (INS) levels and gastric antral cholecystokinin (CCK) content in rats with diabetic gastroparesis (DGP).Methods Sixty SD rats were randomized into groups A, B, C, D and E, 12 rats each. Group A is a normal control. A DGP model was made by an intraperitoneal injection of 2% streptozocin (STZ) plus an irregular high sugar and fat diet in groups B, C, D and E. group B is a model one and did not receive treatment. Group C received electroacupuncture at points Zusanli, Sanyinjiao and Liangmen; group D, electroacupuncture at the control points of Zusanli, Sanyinjiao and Liangmen. Group C, an oral gavage of metoclopramide tablet solution. Blood sugar and urine sugar were determined using a OneTouch lood glucose meter and Tes-Tape, respectively. The gastric emptying rate and the intestinal migration rate were measured by an oral gavage of phenol red. Serum INS levels and gastric antral CCK content were measured by ELISA in every group of rats before and after treatment.Results There were statistically significant differences in blood and urine sugar values between group B, C, D or E rats and group A (P<0.01) and between group C rats and group B or E (P<0.05). There was a statistically significant difference in the gastric emptying rate between group B, C, D or E rats and group A (P<0.01) and between group C rats and group B or D (P<0.01,P<0.05). There was a statistically significant difference in the intestinal migration rate between group B or D rats and group A (P<0.01) and between group C rats and group B or D (P<0.01). There were statistically significant differences in serum INS levels and gastric antral CCK content between groups B and A rats (P<0.05,P<0.01). There was a statistically significant difference in gastric antral CCK content between groups D and A rats (P<0.01). There were statistically significant differences in serum INS levels and gastric antral CCK content between groups C or E rats and group B (P<0.05,P<0.01). There was a statistically significant difference in gastric antral CCK content between groups C and D rats (P<0.05).Conclusions Electroacupuncture can markedly improve gastrointestinal functions and promote gastric emptying in DGP rats. Its therapeutic effect may be related to electroacupuncture raising serum INS levels and reducing gastric antral CCK content.关键词
针刺疗法/电针/胃轻瘫/糖尿病并发症/胰岛素/胆囊收缩素Key words
Acupuncture therapy/Electroacupuncture/Gastroparesis/Diabetic complications/Insulin/Cholecystokinin分类
医药卫生引用本文复制引用
贺凤娥,万全荃,林亚平,彭艳,沈菁..电针对糖尿病性胃轻瘫模型大鼠INS水平和CCK含量的影响[J].上海针灸杂志,2016,(1):81-84,4.基金项目
国家自然科学基金项目(81403487) （81403487）
湖南省高校创新平台开放基金项目(12K088) （12K088）
