现代妇产科进展Issue(12)：893-897,5.DOI:10.13283/j.cnki.xdfckjz.2015.12.004
Dyrk1 B在上皮性卵巢癌组织和细胞中的表达及意义
Expression and significance of Dyrk1 B in the specimens and cells of epithelial ovarian cancer
摘要
Abstract
Objective:To examine the expression of Dyrk1 B in the specimens and cells of epithelial ovarian cancer,and to explore the role of Dyrk1B in the occurrence and develop-ment of ovarian cancer. Methods:Dyrk1B protein was determined by Western blot in ovarian cancer cells treated with/without Dyrk1B inhibitor,AZ191. The cellular proliferation and apop-tosis of ovarian cancer cells after 48 h-treatment of AZ191 were analyzed by MTT assay and Flow cytometry,respectively. Immunohistochemistry was utilized to detect Dyrk1B expression in the specimens of 119 cases of epithelial ovarian cancer,which was included 77 cases of serous car-cinoma,17 cases of mucinous carcinoma,15 cases of endometrial carcinoma, and 10 cases of clear cell carcinoma. Results:Dyrk1B was widely overexpressed in vary kinds of cell lines of OVCAR8, OVCAR5, OVCAR4, OVCAR3, SKOV3, OV2008, C13, A2780S, and A2780CP. Dyrk1 B protein of the ovarian cancer cells was significantly reduced by Western blot after 48 h-treatment of AZ191 . The cellular proliferation was significantly inhibited and cell apoptosis of o-varian cancer cells analyzed by Flow cytometry was significantly increased after 48 h-treatment of AZ191 in a concentration-dependent manner ( P<0 . 05 ) . The immunohistochemical results showed that Dyrk1 B was overexpressed in the specimens of ovarian cancer with the expression rate 90. 8%(108/119),compared with normal ovarian tissue with the expression rate 15. 7%(3/19),which exhibited statistically significant difference (P=0. 001). Conclusion:Dyrk1B is overexpressed in both the specimens and cells of epithelial ovarian cancer. AZ191,an inhibi-tor of Dyrk1B,can inhibit cellular proliferation and induce apoptosis of ovarian cancer cells ac-companied by decreased Dyrk1B protein expression. All together, our study suggests that Dyrk1B may play an important role in the tumorigenesis and development of ovarian cancer,and it may serve as important therapeutic target for the patients.关键词
卵巢癌/Dyrk1 B/细胞凋亡/免疫组化/蛋白印迹法Key words
Ovarian cancer/Dyrk1 B/Apoptosis/Immunohistochemistry/Western blot分类
医药卫生引用本文复制引用
陈婧盈,裴燕,张朋新,庞露,李季,高景春..Dyrk1 B在上皮性卵巢癌组织和细胞中的表达及意义[J].现代妇产科进展,2015,(12):893-897,5.基金项目
国家自然科学基金项目(No:81172457) （No:81172457）
