现代妇产科进展Issue(12)：903-906,4.DOI:10.13283/j.cnki.xdfckjz.2015.12.006
HCG对人子宫内膜细胞VEGF表达调控的初步研究
The preliminary study on the regulation of VEGF by HCG in human endometrium in vitro
摘要
Abstract
Objective:To investigate the mechanism and effection of HCG on the ex-pression of VEGF on human endometrial cells in vitro culture model. Method:Endometrial cells were isolated by mechanical dissociation and centrifugation from endometrial tissue for the pri-mary cells culture, which divided into two groups:Control group and HCG group. The mRNA was analyzed by RT-PCR semi-quantitative. The location of protein was identified by immuno-histochemistry and immunofluorescence. Quantity of proteins was performed by Western blot. Results:The LH/hCG-R and VEGF were detected in cytomembrane and cytoplasm of endome-trium cells by immunohistochemistry respectivity. After HCG intervention we detected VEGF mRNA and protein expression levels at different times and cansure that the optimal stimulation time of HCG acting on VEGF in endometrial cells was 24 hours. Both the mRNA and protein expressions of VEGF were significantly up-regulated in HCG group compared with that in con-trol group ( P<0 . 05 ) ( Q-PCR:1 vs 2 . 0132 ± 0 . 6024;WB:0 . 4069 ± 0 . 1897 vs 0 . 7781 ± 0 . 1651 ) . Conclusion:Successfully established the in vitro culture model of human endometrial cells and completed intervention trials of HCG;HCG up-regulated the gene and protein expres-sion levels of VEGF after combining with HCG/LH-R.关键词
人子宫内膜细胞/HCG/LH/HCG-R/VEGFKey words
Human endometrial cells/HCG/LH/HCG-R/VEGF分类
医药卫生引用本文复制引用
徐少元,李洁,郭鲁燕,陈晓燕,张颖..HCG对人子宫内膜细胞VEGF表达调控的初步研究[J].现代妇产科进展,2015,(12):903-906,4.基金项目
国家自然科学基金资助项目(No:2008-30,872,762) （No:2008-30,872,762）
