中国组织工程研究Issue(50)：8177-8183,7.DOI:10.3969/j.issn.2095-4344.2015.50.025
软基质力学特性对滑膜间充质干细胞向软骨细胞分化的干预作用
Effects of soft substrates on the chondrogenic differentiation of human synovial-derived mesenchymal stem cells
摘要
Abstract
BACKGROUND:Our previous studies have shown that a soft substrate has a significant effect on morphology and cytoskeleton of rat bone marrow mesenchymal stem cel. <br> OBJECTIVE:To explore the effect of polyacrylamide gels as soft substrates with different elastic moduli on the chondrogenic differentiation of human synovial-derived mesenchymal stem cels. <br> METHODS:The synovium was harvested from patients with osteoarthritis under sterile conditions, and primary human synovial-derived mesenchymal stem cels were separated using limiting dilution assay. The flow cytometry and multi-directional differentiation experiments were used to identify the cel surface markers and function of the human synovial-derived mesenchymal stem cels, respectively. The polyacrylamide gels with the elastic modulus of 0.4, 6, 30 kPa, which were made using various amounts of acrylamide and bis-acrylamide, were used to culture human synovial-derived mesenchymal stem cels under induction with transforming growth factor-β1 for 7 and 14 days. RT-PCR was used to test the expression of chondrogenic genes, type II colagen gene and cartilage acidic protein 1. The 6-wel cel culture plates served as controls. <br> RESULTS AND CONCLUSION: The human synovial-derived mesenchymal stem cels showed different cel morphology in the different elastic modulus of polyacrylamide gels. The expression of type II colagen gene and cartilage acidic protein 1 were affected by the different elastic modulus of polyacrylamide gels and culture time, and there was an interaction between these two factors. At 7 days of induction, the expression of cartilage acidic protein 1 gene on 6 kPa polyacrylamide gels was the highest (F=44.350,P=0.000); meanwhile, the expression of type II colagen gene on 0.4 kPa polyacrylamide gels was the highest (F=6.384,P=0.005). These findings indicate that polyacrylamide gels with lower elastic modulus are superior to routine culture plates to promote the chondrogenic differentiation of human synovial-derived mesenchymal stem cels.关键词
干细胞/分化/软基质/聚丙烯酰胺凝胶软基质/弹性模量/转化生长因子β1/滑膜间充质干细胞/单细胞克隆/软骨细胞Key words
Synovial Membrane/Mesenchymal Stem Cels/Acrylamides/Elastic Modulus/Transforming Growth Factor beta1/Chondrocytes/Cel Differentiation/Tissue Engineering分类
医药卫生引用本文复制引用
崔爽爽,余兆镇,于顺禄,赵文君,赵丽坤,邢国胜,段小圆..软基质力学特性对滑膜间充质干细胞向软骨细胞分化的干预作用[J].中国组织工程研究,2015,(50):8177-8183,7.基金项目
天津市卫生局科技基金面上项目(2012KY23,2012KY25)Funding：the Science and Technology Foundation of Tianjin Municipal Health Bureau, No.2012KY23,2012KY25 （2012KY23,2012KY25）
