中国组织工程研究Issue(50)：8184-8189,6.DOI:10.3969/j.issn.2095-4344.2015.50.026
血小板CD36新等位基因1142T>G序列分析及确认
Analysis and identification of a novel CD36 allele, 1142 T>G
摘要
Abstract
BACKGROUND:As a main antigen of platelet, CD36 antigen is also known as platelet glycoprotein IV (GPIV). The mutation of CD36 gene may result in deficiency of the antigen. <br> OBJECTIVE:To identify a novel CD36 alele. <br> METHODS: DNA was isolated from peripheral blood sample, and 12 coding regions of CD36 gene were amplified by PCR. Sequencing-based typing was used to analyze the sequence of the target regions. The derived sequences were aligned with the standard sequence of NG_008192 in GenBank to identify the novel alele. <br> RESULTS AND CONCLUSION: 1142 T>G mutation was detected in exon 12 of CD36 gene of the proband, and the other regions were consistent with the standard sequence. No data or report about 1142 T>G was found in GenBank or National Center for Biotechnology Information (NCBI), and thus it was reported to GenBank and received by number KM275213. 1142 T>G results in amino acid 381 Leu>Ser of the CD36 protein. There is a big difference in hydrophilia and polarity of the two amino acids. Also the 381 amino acid locates in highly conserved region. Thus it is speculated that 1142 T>G may reduce or vanish the activity of the protein.关键词
干细胞/组织工程/新等位基因/CD36基因/基因突变/1142 T>G/序列分析Key words
DNA Mutational Analysis/Aleles/Sequence Analysis分类
医药卫生引用本文复制引用
林凤秋,李晓丰,邵超鹏,李剑平..血小板CD36新等位基因1142T>G序列分析及确认[J].中国组织工程研究,2015,(50):8184-8189,6.基金项目
沈阳市科学技术计划项目(F13-220-9-16)Funding：the Science and Technology Foundation of Shenyang, No. F13-220-9-16 （F13-220-9-16）
