中国动物检疫Issue(1)：26-29,4.
奶牛疣状毛癣菌的分离鉴定及其耐药性分析
Isolation and Identiifcation ofTrichophyton Verrucosum in Cows and Analysis on Vitro Drug Resistance
摘要
Abstract
In order to explore the dermatophyte situation of the dairy cows and the antifungal susceptibility,the hair and skin specimens were collected from the skin lesions of Holstein cows with the typical ringworm symptoms. The pathogens were isolated and identified through ribosome DNA ITS sequence analysis. The MIC of 5-fluorocytosine(5-FC),amphotericin B(AMB),fluconazole(FCA),itraconazole(ITR)and voriconazole(VRC)were determined by ATB FUNGUS 3. The results showed that the isolated fungi all showed waxy raised center,firtree periphery. The fungi were identified to be the zoonotic dermatophyte-Trichophyton verrucosum by ITS sequence analysis. The isolatedTrichophy-ton verrucosum was sensitive to ITR and VRC,intermediate to FCA,and resistant to 5-FC and AMB.Trichophyton ver-rucosum could be identified quickly and accurately by rDNA ITS sequence analysis. ITR and VRC demonstrated it had higher vitro antifungal activity.关键词
皮肤真菌病/疣状毛癣菌/ITS序列/耐药性/人兽共患/分离鉴定Key words
dermatophytosis/Trichophyton verrucosum/ITS sequence/drug resistance/Zoonosis/isolation and identification分类
农业科技引用本文复制引用
王建昌,姜彦芬,王珅,王金凤..奶牛疣状毛癣菌的分离鉴定及其耐药性分析[J].中国动物检疫,2016,(1):26-29,4.基金项目
河北出入境检验检疫局科研项目 （）
