中国动物检疫Issue(1)：71-75,5.
蓝耳病病毒抗体ELISA检测试剂盒的“两步法”评价
Evaluation of ELISA Kits for PRRSV Antibody Detection by ‘Two Steps’ Method
摘要
Abstract
This study aimed at evaluating four commercial ELISA kits for PRRSV antibody detection by using two-steps for the first time. Firstly,18 reference sera were used to screen. Secondly,6 reference sera were used to detect the limitation of detection(LOD)and repeatability(Re),and 343 field samples were used to test sensitivity and specificity. The results showed that 4 kits were 100% identical to the reference seras in screening test in the first step,and the sensi-tivity and specificity of A,B,C and D were 94.58% and 97.14%,94.58% and 89.2%,93.1% and 90%,96.55% and 85%respectively in the second step. Kit A and B are universal kits for detecting antibody against EU and US type of PRRSV. Kit C and D can only detect US type virus antibody. D kit showed coefficient of variation was low among the batches and had good repeatability. The 4 PRRSV kits were varying in detection scale,validity and accuracy. By applying two-step method,this study supplied evidences for the selection of PRRSV antibodies ELISA detection kits,also gave the reference for the evaluation of animal disease detection.关键词
猪繁殖与呼吸综合征/ELISA检测试剂盒/诊断敏感性/诊断特异性/重复性Key words
PRRS/commercial ELISAdiagnostic kit/diagnostic sensitivity/diagnostic specificity/diagnostic repeat-ability分类
农业科技引用本文复制引用
王勤,林祥梅,刘晓飞,冯春燕,翟新验,周智,吴绍强,李晓琳,仇松寅,刘丹丹..蓝耳病病毒抗体ELISA检测试剂盒的“两步法”评价[J].中国动物检疫,2016,(1):71-75,5.基金项目
国家质检总局科技计划项目猪蓝耳病抗体检测试剂盒贝叶斯方法质量评价模型的建立（2014IK230）；中国检验检疫科学研究院基本科研业务费专项资金资助项目动物检疫风险分析中关键参数的精准估计（2014JK030）；国家质检总局科技计划项目《进境高危害病毒性猪病检测方法的系统性研究和完善》 （）
