中国感染控制杂志Issue(12)：793-797,5.DOI:10.3969/j.issn.1671-9638.2015.12.001
HMGB1抗体对刀豆蛋白A引起小鼠肝损伤的保护作用
Protective role of high mobility group box-1 protein antibody in ConA-in-duced liver injury in mice
摘要
Abstract
Objective To detect the protective role of high mobility group box-1 protein (HMGB1 )antibody in concanavalin A(ConA)-induced liver injury in mice.Methods The healthy male Balb/c mice were grouped into con-trol group (saline injection),model group(ConA injection)and experimental group(ConA+HMGB1 antibody injec-tion).After 6 hours of injection,mice blood was collected for detecting alanine transaminase (ALT)and HMGB1 , liver tissue was used to do HE stain,Tunel,and immunofluorescence detection.Results Pathological inflammation in experimental group was slighter than model group.The levels of ALT and HMGB1 in mice serum were (52.00± 8.34)U/L and (7.54 ±0.53)ng/mL in control group,(5 551 .50 ±1 445.74)U/L and (18.06 ±1 .65 )ng/mL in model group,(1 977.40±654.89)U/L and (10.77±0.71)ng/mL in experimental group,respectively;the expres-sion levels of HMGB1 mRNA and HMGB1 (relative value)in liver tissue were 1 .886±0.253 and 0.086±0.028 in control group,4.718±0.341 and 0.268±0.043 in model group,3.005 ±0.331 and 0.116±0.008 in experimental group,respectively;the expression levels of ALT and HMGB1 in serum,as well as HMGB1 mRNA and HMGB1 in liver tissue of experimental group were all lower than model group(all P <0.001).Apoptosis and HMGB1 migra-tion in the liver cell (normalized)were 1 ±0 and 1 ±0 in control group,4.67 ±0.33 and 4.50 ±0.22 in model group,2.67±0.21 and 2.33 ±0.21 in experimental group,respectively;apoptosis and HMGB1 migration in liver tissue of experimental group were both lower than model group(both P <0.001).Conclusion HMGB1 antibody can improve the pathological injury of liver tissue,and protect mice liver against the injury induced by ConA.关键词
HMGB1抗体/高迁移率族蛋白1/刀豆蛋白A/肝损伤/保护作用/肝损伤模型/急性肝损伤/模型小鼠Key words
HMGB1 antibody/high mobility group box-1/concanavalin A/liver injury/protective role/liver in-jury model/acute liver injury/mouse model分类
医药卫生引用本文复制引用
黄泽炳,黄燕,周蓉蓉,陈若蝉,易盼盼,李宁,范学工..HMGB1抗体对刀豆蛋白A引起小鼠肝损伤的保护作用[J].中国感染控制杂志,2015,(12):793-797,5.基金项目
国家国际科技合作专项（2015DFA31490）；国家自然科学基金（81272253、30972621）；湖南省科技厅国际科技合作重点项目（2012WK2001）；湖南省科技厅计划项目（2014SK4066）；湖南省自然科学基金项目（11JJ6072）；湖南省科技厅课题 （）
