中国临床药理学杂志Issue(24)：2430-2432,3.DOI:10.13699/j.cnki.1001-6821.2015.24.019
高效液相色谱－质谱联用法测定人血浆中左舒必利的浓度
Pharmacokinetics study of levosulpiride in human plasma by HPLC-MS/MS
华雯妍 1张全英 1宗顺麟 1黄明 1王蒙1
作者信息
- 1. 苏州大学 附属第二医院 临床试验机构，江苏苏州 215004
- 折叠
摘要
Abstract
Objective To establish an HPLC-MS/MS method for de-termination of levosulpiride in human plasma.Methods After protein precipitation, the plasma was separated on Xterra ® RP18 (4.6 mm × 150 mm, 5 μm ) column, with a mobile phase of acetonitrile -10 mmoL・ L-1 ammonium acetate containing 0.2% formic acid ( 10∶90 ) . The flow rate was 1.0 mL・ min -1 and the column temperature was 30℃.Quantification was performed in the positive ion multiple reaction mo-nitoring( MRM) mode.Results Determination of levosulpiride had good linearity in the concentration range of 1.00 -600.00 μg・ L-1 .The standard curve was y=2.93 ×10 -3 x+1.75 ×10 -2 ( n=3,r=0.999 3). The lower limit of quantization was 1.00 μg・ L-1 , extraction recovery rate was 92.12%-98.53%, and intra -batch and inter-batch RSD were both less than 4.61%. Conclusion The method is specific, simple, sensitive, rapid, accurate and suitable for determination of levosulpiride in human plasma.关键词
左舒必利/高效液相色谱-质谱联用/药代动力学Key words
levosulpiride/HPLC-MS/MS/pharmacokinetics分类
医药卫生引用本文复制引用
华雯妍,张全英,宗顺麟,黄明,王蒙..高效液相色谱－质谱联用法测定人血浆中左舒必利的浓度[J].中国临床药理学杂志,2015,(24):2430-2432,3.
