中国实验动物学报Issue(6)：567-572,6.DOI:10.3969/j.issn.1005-4847.2015.06.004
冷驯化对中缅树鼩褐色脂肪组织适应性产热的影响
Adaptive thermogenesis of the brown adipose tissue in tree shrews (Tupaia belangeri) during cold acclimation
摘要
Abstract
Objective To investigate the effect of ambient temperature on body mass, thermogenic activity and un-coupling protein-1 ( UCP1) content of brown adipose tissue ( BAT) in tree shrews ( Tupaia belangeri) , and to provide the-oretical basis for establishing tree shrews model of obesity.Methods Forty healthy adult tree shrews with similar body mass were uesd in our experiment.The tree shrews were divided into five groups (n=8):control group (0 d), the ani-mals were maintained under 25 ±1℃ and 12L:12D ( light : dark, lights on 08:00) photoperiod; and the animals were maintained under 5 ±1℃and 12L:12D photoperiod for 7 d, 14 d, 21 d and 28 d groups, respectively.At the end of ex-periment, the changes of body mass, nonshivering thermogenesis (NST), BAT mass and uncoupling protein 1 (UCP1) con-tent were determined.Results Compared with the control group (0 d), the body mass, NST, BAT mass and UCP1 con-tent of the cold acclimation groups were improved significantly, the BAT color also obviously deepened, and after cold accli-mation for 28 d, the body mass, NST, BAT mass and UCP1 content were increased by 26.32%, 20.65, 53.85%and 43%, respectively.Apparently, the UCP1 content was significantly positively correlated with BAT mass and NST.Conclusions BAT proliferation may be induced and UCP1 expression upregulated by cold acclimation in Tupaia belangeri, therefore, en-hancing the thermogenic activity of brown adipose tissue to increase energy expenditure.We would speculate that BAT might be used as a target organ for treatment of obesity by energetic approach in the future.关键词
中缅树鼩/冷驯化/非颤抖性产热/褐色脂肪组织/解偶联蛋白1/肥胖/产热Key words
Tupaia belangeri chinensis/Cold acclimation/Nonshivering thermogenesis ( NST)/Brown adipose tissue(BAT)/Uncoupling protein 1( UCP1)/Obesity/Thermogenesis分类
生物科学引用本文复制引用
高文荣,曹能,朱万龙,张浩,王政昆,陈金龙..冷驯化对中缅树鼩褐色脂肪组织适应性产热的影响[J].中国实验动物学报,2015,(6):567-572,6.基金项目
国家科技支撑计划项目子课题（编号2014BAI01B01－06）；国家国际科技合作专项项目（编号2014DFR31040）；国家自然科学基金项目（编号No．31360096）；云南省博士研究生学术新人奖资助。 （）
