大鼠椎间盘髓核细胞体外凋亡模型的建立OA北大核心CSCDCSTPCD
Establishment of an apoptosis model of rat disc nucleus pulposus cells in vitro
目的：建立大鼠椎间盘髓核细胞体外凋亡模型。方法为了充分模拟退变椎间盘内营养缺乏的微环境，本实验分别采用含1％，3％，5％，8％，10％胎牛血清的DMEM培养基培养椎间盘髓核细胞，筛选最佳促凋亡浓度，分别检测髓核细胞凋亡率、凋亡相关蛋白Bax、bcl－2、caspase－3酶的表达、细胞增殖曲线及免疫荧光分析。结果流式细胞仪测得髓核细胞凋亡率随着胎牛血清（ FBS）浓度降低而升高，3％FBS为最有效诱导凋亡浓度；Western blot示Bax、c…查看全部>>
Objective To develop an apoptosis model of nucleus pulposus cells in cell culture.Methods To mimic the nutrient-deficient microenvironment of degenerative intervertebral disc,nucleus pulposus cells derived from infant SD rat disc were cultured under serum limiting conditions.Nucleus pulposus cells were cultured in culture medium contai-ning 1%, 3%, 5%, 8%and 10%fetal bovine serum( FBS) respectively to select the optimum FBA concentration.Apoptosis was assess…查看全部>>
王厚磊;卢伟;李德芳;丁磊;吴靖平
复旦大学附属金山医院骨科，上海 201508复旦大学附属金山医院骨科，上海 201508复旦大学附属金山医院骨科，上海 201508复旦大学附属金山医院骨科，上海 201508复旦大学附属金山医院骨科，上海 201508
生物科学
椎间盘退变髓核细胞凋亡营养夺获大鼠
Intervertebral disc degenerationNucleus pulposus cellsApoptosisNutrition deprivationRat
《中国实验动物学报》 2015 (6)
607-611,5
上海市卫生局科研项目（编号2012－341）。
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