中国实验动物学报Issue(6)：622-627,6.DOI:10.3969/j.issn.1005-4847.2015.06.015
小鼠脐带间充质干细胞的体外诱导分化
Isolation and differentiation in vitro of mouse umbilical cord mesenchymal stem cells
摘要
Abstract
Objective To isolate, culture and identify mouse umbilical cord mesenchymal stem cells( mUCMSCs) and to study whether they can be induced to differentiate into adipocytes, chondrocytes and osteoblasts.Methods The mUCMSCs were isolated and expanded by adherent culture from fresh mouse umbilical cord.The morphological characteris-tics of the resulting cells were observed under inverted phase contrast microscope, and their expression of mesenchymal sur-face markers was identified and analyzed by flow cytometry.Then mUCMSCs were induced to differentiate into chondro-cytes, adipocytes and osteoblasts in vitro.Results Fibroblast-like cells could be isolated from the fresh umbilical cord by adherent culture.These adherent cells highly expressed mesenchymal markers including CD29, CD90 and CD105 while low expression of CD34.The cells were successfully induced to differentiate into chondrocytes, adipocytes and osteoblasts. Conclusions The mUCMSCs isolated from fresh mouse umbilical cord by adherent culture have potential of differentiation into chondrocytes, adipocytes and osteoblasts.关键词
小鼠/脐带间充质干细胞/细胞培养/成软骨诱导/成脂诱导/成骨诱导Key words
Mouse/Umbilical cord mesenchymal stem cells/Cell culture/Chondrocytes induction/Adipocytes induction/Osteoblast induction分类
生物科学引用本文复制引用
朱慧,叶莉,何洁,余璞,王金祥,王强,赵晶,庞荣清,白杰英..小鼠脐带间充质干细胞的体外诱导分化[J].中国实验动物学报,2015,(6):622-627,6.基金项目
云南省基金项目（2011HB050，2013DA004）；十二五重大项目（2011ZXJ09201－031）；国家自然科学基金（31272385）；北京市自然科学基金（5152023）。 （）
