中国实验动物学报Issue(6)：643-647,5.DOI:10.3969/j.issn.1005-4847.2015.06.019
近红外荧光染料在胃癌人源性肿瘤组织异种移植模型研究中的应用
Application of near infrared fluorescent dye in the study of patient-derived gastric cancer xenograft nude mouse models
摘要
Abstract
Objective To establish a patient-derived gastric cancer xenograft( PDX) model in nude mice and to in-vestigate the application of near infrared fluorescent ( NIRF) dye IR-783 in in vivo imaging of gastric cancer xenograft mod-els.Methods Fresh human gastric cancer tissue was taken and transplanted into the subrenal capsule of nude mice to es-tablish the xenograft model.When the transplanted tumors grew,took part of the tumor tissue to do HE staining and compare the structural characteristics with the primary tumor.Another portion of the tumor was xenografted into nude mice subcutane-ously.Twenty days later,the tumor-bearing mice were injected intraperitoneally with IR-783 dye (10μM) in a dose of 100 mg/20 g.The intensity of the tumor image was monitored by optical NIRF imaging.The correction between tumor volume and fluorescence intensity was analyzed.Finally,the expression of OATP1B3 and HIF1αin the xenografted tumor tissue was detected by immunohistochemistry.Results We successfully established three patient-derived xenograft ( PDH) models of human gastric cancer.The transplanted tumor tissues maintained the histological characteristics of the primary tumor well.NIRF signal can be detec ted in subrenal capsule of the xenografted nude mice.The correlation between tumor size and fluorescence intensity in the PDX models reached higher than 98%.Strong positive expressions of HIF1αand OATP1B3 in the tumor tissues were detected.Conclusions NIRF dye IR-783 can be specifically accumulated at the tumor site,therefore, can be used to detect PDX in vivo early.The tumor targeting property may be related to the expression of OATP1B3 and HIF1α.关键词
人源性肿瘤组织异种移植模型/近红外荧光染料/低氧诱导因子-1/有机阴离子转运多肽1B3Key words
Patient-derived tumor xenograft( PDX) model/IR-783/HIF1α/OATP1B3/Nude mice分类
生物科学引用本文复制引用
赵宁宁,张彩勤,赵勇,杨丽,张海,刘漪沦,师长宏..近红外荧光染料在胃癌人源性肿瘤组织异种移植模型研究中的应用[J].中国实验动物学报,2015,(6):643-647,5.基金项目
军队实验动物专项课题资助（SYDW2014－002）；国家自然科学基金项目（31572340）。 （）
