中国比较医学杂志Issue(12)：59-64,6.DOI:10.3969.j.issn.1671-7856.2015.12.012
SHR 大鼠前额叶、纹状体突触体的提取方法
An optimized extraction protocol of prefrontal cortical and striatal synaptosomes from SHR rat
摘要
Abstract
Objective To introduce an improved extraction method of prefrontal cortical and striatal synaptosomes from SHR rat. Methods Synaptosomes were prepared from SHR rat brain tissue by Percoll density gradient centrifugation.Transmission electron microscopy was used to assess the morphology and structural integrity of the synaptosomes.Results The obtained synaptosomes showed oval structures surrounded by an intact membrane.Presynaptic components contained one or more mitochondria and a large number of synaptic vesicles.The synaptic clefts were clearly visible, and prominent part of the characteristic compact structure was clear, complete and with higher electron-density. The synaptosome presynaptic membrane, synaptic cleft, and postsynaptic membrane were well preserved, and the synaptosomes were densely distributed, showing typical morphological characteristics of synaptosomes.Conclusions The results of our study improved the traditional preparation method and provide a less time-consuming, highly productive protocol for preparation of structurally typical and intact synaptosomes, suitable for further research on neuroscience and neurological diseases.关键词
Percoll/密度梯度离心法/SHR大鼠/前额叶/纹状体/制备Key words
Synaptosome/Percoll/Density gradient centrifugation/SHR mice/prefrontal cortex/Striatum分类
医药卫生引用本文复制引用
周荣易,韩新民,王娇娇,孙继超..SHR 大鼠前额叶、纹状体突触体的提取方法[J].中国比较医学杂志,2015,(12):59-64,6.基金项目
国家自然科学基金（81273801）安神定志灵调控ADHD多巴胺D1／D2受体信号通路及脱敏／复敏的机制研究。 （）
