中国医科大学学报Issue(12)：1061-1065,5.
Beclin 1表达在胃癌发生和演进中的临床病理意义及作用机制
Clinicopathological Significance and Relevant Molecular Mechanisms of Beclin 1 in Gastric Cancer
摘要
Abstract
Objective to explore the role of Beclin 1 in gastric carcinogenesis and subsequent progression. Methods MkN28 cells were trans-fected with Beclin 1-expressing plasmid,and then the proliferation and cell cycle was measured by CCk-8 and PI staining. Beclin 1 expression was examined by immunohistochemistry and in situ hybridization on tissue microarrays containing gastric cancers,adjacent non-neoplastic mucosa,and metastatic lymph node. the correlation with the tumorgenesis,clinicopathological and prognostic parameters was analyzed. Results Beclin 1 overex-pression resulted in G2 arrest of MkN28 cells and reduced the proliferation. Beclin 1 mRNA was highly expressed in gastric cancer than matched mu-cosa by ISH(P < 0.05). Beclin 1 was highly expressed in male than female patients with gastric cancer(P < 0.05). the elder patients with gastric cancer had higher Beclin 1 expression than younger ones(P < 0.05). the diffuse-type carcinomas showed less Beclin 1 expression than intestinal and mixed type ones(P < 0.05). kaplan-Meier analysis indicated that Beclin 1 expression was positively correlated to favorable prognosis of the can-cer patients(P < 0.05). Conclusion Beclin 1 expression is closely linked to pathogenesis,metastasis and differentiation of gastric cancer. Beclin 1 might be employed to indicate the favorable prognosis of gastric cancer patients and regarded as a target of gene therapy.关键词
胃癌/Beclin 1/疾病发生/演进/预后Key words
gastric cancer/Beclin 1/pathogenesis/progression/prognosis分类
医药卫生引用本文复制引用
郑华川,申道福,杨雪峰,时帅,高阳,赵爽,张文陆..Beclin 1表达在胃癌发生和演进中的临床病理意义及作用机制[J].中国医科大学学报,2015,(12):1061-1065,5.基金项目
国家自然科学基金（81172371，81472544）；辽宁省教育厅优秀人才计划（LJQ2014093）；辽宁省科学技术厅2012年博士启动资助项目（20121095）；辽宁医学院2012年博士启动项目（Y2012B018）；辽宁医学院校长基金 （）
