中国医科大学学报Issue(12)：1070-1074,5.
白色念珠菌感染对脾虚模型小鼠T细胞亚群的影响
Influence of Candida albicans Infection on T Cell Subsets in the Mice with Spleen Deficiency
摘要
Abstract
Objective to observe the influence of Candida albicans infection on t cell subsets in mice with spleen deficiency,so as to explore the immune mechanism. Methods totally 150 healthy SPF mice were randomly divided into four groups:control group(N1 group,n = 30);Candida albicans infection group(N2,n = 40),spleen deficiency model group(M1,n = 40),spleen deficiency model combined with Candida albicans in-fection group(M2,n = 40). N2 and M1 mice were infected by Candida albicans at a concentration of 2×108 CFU/mL. ten mice were randomly se-lected from each group at 7,14,and 21 days after infection respectively,and the index was detected. Flow cytometry was used to detect the percent-age of CD4+/CD8+t cells in intestinal mucosa of mice,and the expression level of IL-4 and IFN-γ mRNA was detected by Rt-PCR assay. the levels of IFN-γ and IL-4 were detected by Western blot assay. Results Compared with N1 group,the proportion of CD4+t cells in the lamina of the natu-ral layer of the small intestine was decreased(P < 0.01),the proportion of CD8+t cells was increased(P < 0.01),the ratio of the two was significant-ly decreased(P < 0.01),and the expression levels of IL-4,mRNA IFN-γ and protein in the small intestine tissues were increased in other groups (P < 0.01). In addition,the expression level of IFN-γ was significantly increased in M2 group,while the expression of IL-4 was significantly in-creased in N2 group. Conclusion the susceptibility of Candida albicans infection was increased in spleen deficiency mice,which may be closely related to the regulation of th1/th2 balance.关键词
脾虚证/白色念珠菌/感染/t细胞亚群Key words
spleen deficiency/Candida albicans/infection/t lymphocyte subsets分类
医药卫生引用本文复制引用
马贤德,张威,关洪全..白色念珠菌感染对脾虚模型小鼠T细胞亚群的影响[J].中国医科大学学报,2015,(12):1070-1074,5.基金项目
国家自然科学基金 （）
