中国药理学通报Issue(1)：27-32,6.DOI:10.3969/j.issn.1001-1978.2016.01.007
4-氨基-2-三氟甲基苯基维甲酸酯对人白血病 K562细胞的蛋白质组学研究
The proteomics research of 4-amino-2-trifluoromethyl-phenyl retinate on human leukemia K562 cells
摘要
Abstract
Aim To explore the proteomics mechanism of the differentiation induction effect of 4-amino-2-trif-luoromethyl-phenyl retinate(ATPR)on human leukemi-a K562 cells. Methods Human leukemia K562 cells were incubated with the same concentration (1 × 10 - 6 mol·L - 1 ) of ATPR or ATRA for 48 hours. The total cell proteins were collected, purified and digested by trypsin, solid phase extraction, and the peptides were detected by ESI-LC-MS / MS. The difference of the pro-tein expression between the cells treated with ATPR and ATRA was compared by using the Discoverer Pro-teome 1. 2 software, and the molecular function, the biological process and other information of those pro-teins were analyzed based on the DAVID, KEGG, STRING databases. Results 120 specific proteins were identified only in the ATPR group, 143 only in the ATRA group, and 422 other proteins in both groups. Results of DAVID analysis showed that ATPR-induced specific proteins were mainly involved in 39 biological processes of proteins and macromolecules metabolism, protein transport and localization and so on. Results of KEGG analysis revealed that ATPR-in-duced proteins participated in signal pathways, mainly metabolic pathways, PI3K-Akt signal pathway, TGF-beta signal pathway and other pathways in cancer. String protein interaction network analysis displayed that ATPR-induced proteins, like EIF3A, EIF6, RPL3, RPL8, RPL13, RPL7A, RPL21, RPS3, RPS14, NACA, BTF3, NHP2L1, PPP2CA proteins had direct interactions with more than or equal to 10 associated proteins. Conclusion The differentiation induction effect of ATPR on K562 cells might be as-cribed to the ATPR-induced proteins interaction net-work and the specific central proteins it induced, which are involved in the regulation of cell prolifera-tion, differentiation and apoptosis.关键词
4-氨基-2-三氟甲基苯基维甲酸酯/全反式维甲酸/人白血病 K562 细胞/蛋白质组学/抑制增殖/诱导分化Key words
ATPR/ATRA/human leukemia K562 cells/proteomics/inhibition of proliferation/induced differentiation分类
医药卫生引用本文复制引用
孟遥,张冬玲,夏泉,葛金芳,陈飞虎..4-氨基-2-三氟甲基苯基维甲酸酯对人白血病 K562细胞的蛋白质组学研究[J].中国药理学通报,2016,(1):27-32,6.基金项目
国家科技部“重大新药创制”科技重大专项( No 2011 ZX09401-021) （ No 2011 ZX09401-021）
