中国药理学通报Issue(1)：33-36,37,5.DOI:10.3969/j.issn.1001-1978.2016.01.008
胆汁酸膜受体 TGR5对高糖培养的大鼠肾小球系膜细胞 FN、TGF-β1的调控作用
Regulatory effects of the bile acid membrane receptor TGR5 on FN and TGF-β1 in rat glomerular mesangial cells cultured under high glucose condition
摘要
Abstract
Aim To investigate the expression of G protein-coupled receptor TGR5 and its effects on FN and TGF-β1 expression cultured under high glucose condition in rat glomerular mesangial cells , and then to explore the role of TGR5 in diabetic nephropathy. Methods INT-777 and TGR5 plasmid were used to activate TGR5 under high glucose(HG,30 mmol·L - 1 glucose ) condition, and anti-TGR5 small interfering RNA(TGR5 siRNA) was used to knock down TGR5. The protein expression of FN and TGF-β1 in rat me-sangial cells was detected by Western blot. Results TGR5 could be detected in rat glomerular mesangial cells. Both FN and TGF-β1 protein levels could be in-creased by high glucose compared with control group(P < 0. 05),and be inhibited by activiation of TGR5(P <0. 05). On the other hand,knockdown of TGR5 could increase FN and TGF-β1 protein to abnormal levels(P< 0. 01,P < 0. 05). Conclusion TGR5 suppresses HG-induced FN and TGF-β1 expression in rat glomer-ular mesangial cells,suggesting a protective role in the process of diabetic nephropathy.关键词
G 蛋白偶联受体/TGR5/高糖/肾小球系膜细胞/纤维连接蛋白/转化生长因子/INT - 777/糖尿病肾病Key words
G protein-coupled receptor/TGR5/high glucose/glomerular mesangial cells/fibronectin/transfor-ming growth factor-β1/INT-777/diabetic nephropathy分类
医药卫生引用本文复制引用
熊凤霄,杨志英,王少贵,陈诚,黄河清..胆汁酸膜受体 TGR5对高糖培养的大鼠肾小球系膜细胞 FN、TGF-β1的调控作用[J].中国药理学通报,2016,(1):33-36,37,5.基金项目
国家自然科学基金资助项目(No 81373457) （No 81373457）
教育部博士点基金资助项目(No 20130171110097) （No 20130171110097）
广东省自然科学基金资助项目(No S2013010015765,S2012020010991) （No S2013010015765,S2012020010991）
