中国药理学通报Issue(1)：43-48,6.DOI:10.3969/j.issn.1001-1978.2016.01.010
灰毡毛忍冬花蕾提取物及其两种主要皂苷的溶血性研究
On hemolysis of extracts of Lonicera Macranthoides flower bud and two saponins
摘要
Abstract
Aim To figure out whether Lonicera mac-ranthoides could induce hemolysis. Methods In vitro, macroscopic observation and spectrophotometry were used to observe whether the solutions of extracts from Lonicera macranthoides, MacranthoidinB and Dipsa-cosideB could induce hemolysis in 2% red cell suspen-sion of New Zealand white rabbits. And the three test-ed materials were prepared in concentration gradient of 5, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100 mg· L - 1; and in vivo, mice were respectively treated with MacranthoidinB (0. 110 g·kg - 1 , 0. 055 g·kg - 1 ), DipsacosideB(0. 020 g·kg - 1 , 0. 010 g·kg - 1 ), ex-tracts (2. 275 g·kg - 1 , 1. 137 g·kg - 1 , crude drugs) once per day for 7 days, and all of the tested doses de-pended on the clinical doses. Then, RBC, RET and MCHC before and after administration were tested. Re-sults The hemolytic ratio in each treated group was below 5% in vitro. And in vivo, the three materials did not induce hemolysis and had no significant influence on RBC,RET and MCHC(P > 0. 05). Conclusions Extracts from flower bud of Lonicera macranthoides, MacranthoidinB and DipsacosideB, have not caused hemolysis in vivo and in vitro in this research.关键词
灰毡毛忍冬花蕾/灰毡毛忍冬皂苷乙/川续断皂苷乙/溶血/肉眼观察法/分光光度法Key words
flower bud of Lonicera macranthoides/MacranthoidinB/DipsacosideB/hemolysis/visual in-spection/spectrophotometry test分类
医药卫生引用本文复制引用
汪宏锦,王红玉,何然,吴俊杰,徐晓玉..灰毡毛忍冬花蕾提取物及其两种主要皂苷的溶血性研究[J].中国药理学通报,2016,(1):43-48,6.基金项目
重庆市应用开发计划项目( No cstc2014yykkfA80023) （ No cstc2014yykkfA80023）
重庆市121科技示范工程(No cstc2014fazktjcsf80038) （No cstc2014fazktjcsf80038）
秀山金银花产业开发项目。 （）
