中国药理学通报Issue(1)：49-54,6.DOI:10.3969/j.issn.1001-1978.2016.01.011
紫草素对脑胶质瘤干细胞干性维持的相关研究
Effects of shikonin on stemness maintance of glioma stem cells
摘要
Abstract
Aim To explore the effect of shikonin on stemness maintance of glioma stem cells ( GSCs ). Methods After the U87-MG cells were cultured and isolated, the sphere cells were identified by immuno-fluorescent staining. The alteration of stemness of GSCs by shikonin treatment(2 μmol·L - 1 ) for 12 h, 24 h and 48 h was valued by morphological detection using optical microscope and sub-sphere forming assay. Mo-reover, the related markers of stem cells, such as CD133, were detected in shikonin treated GSCs by western blot assay. Protein expression of PI3K, p-PI3K, Akt and p-Akt was detected by western blot af-ter shikonin treatment alone. Furthermore, by combi-nation with insulin-like growth factor-1 ( IGF-1), we observed the alteration of stemness maintance of shiko-nin-treated GSCs. Results The presence of neural stem cell related markers CD133 and nestin proved the characteristics of GSCs. Shikonin treatment significant-ly inhibited the morphology of GSCs and the sub-sphere forming. Besides, the reduced expression of CD133 was detected in shikonin treated GSCs. Though, the expression of PI3K and Akt did not change compared with the control group, the expression of p-PI3K and p-Akt was reduced. Furthermore, the combination of IGF-1 markedly attenuated the inhibitory effect of shikonin on stemness maintance of GSCs. Conclusion The stemness maintance of GSCs can be significantly inhibited by shikonin treatment, in which PI3K/ Akt pathway is involved.关键词
紫草素/胶质瘤干细胞/干性维持/PI3K/Akt 信号通路/胰岛素样生长因子-1/自我更新能力Key words
shikonin/glioma stem cells/stemness ma-intance/PI3K/ Akt pathway/insulin-like growth factor-1(IGF-1)/self-renewing ability分类
医药卫生引用本文复制引用
刘静,笪祖科,李振,薛一雪,刘丽波,王萍,刘云会..紫草素对脑胶质瘤干细胞干性维持的相关研究[J].中国药理学通报,2016,(1):49-54,6.基金项目
国家自然科学基金资助项目(No 81502179,81372484,81372682,81172197) （No 81502179,81372484,81372682,81172197）
