中国药理学通报Issue(1)：69-73,5.DOI:10.3969/j.issn.1001-1978.2016.01.015
依普利酮下调 SGK-1表达抑制梗阻性肾病细胞增殖的研究
Inhibitory effects of eplerenone on cell proliferation via down-regulated SGK-1 pathway in rats with unilateral ureteral obstruction
摘要
Abstract
Aim To observe the effect of mineralocor-ticoid receptor blockade eplerenone on cell proliferation in obstructed kidney of rats. Methods Renal intersti-tial fibrotic animals were made with unilateral ureteral obstruction (UUO) and treated with eplerenone100 mg · kg - 1 · d - 1 . The kidneys were harvested on the 10th day and proliferating cell nuclear antigen ( PC-NA ), serum and glucocorticoid induced kinase-1 (SGK-1 ) and transforming growth factor-β1 ( TGF-β1 ) were detected with immunohistochemistry and Western blot. Results Renal histopathology showed large quantities extracellular matrix (ECM) accumula-tion in kidney with UUO, large numbers of inflammato-ry cells infiltrated in renal interstitium, renal tubular expansion and exfoliation of epithelial cells . The cell proliferation and ECM accumulation were inhibited in eplerenone treated rats significantly. Immunohisto-chemistry and Western blot showed that expressions of PCNA,SGK-1 and TGF-β1 were significantly up-regu-lated with UUO and down-regulated by eplerenone. Conclusion Eplerenone plays the role in inhibiting the cell proliferation and reducing ECM accumulation by down-regulating expression of SGK-1 pathway in rats with unilateral ureteral obstruction.关键词
依普利酮/肾间质纤维化/细胞增殖/UUO/PCNA/SGK-1/TGF-β1Key words
eplerenone/renal interstitial fibrosis/cell proliferation/UUO/PCNA/SGK-1/TGF-β1分类
医药卫生引用本文复制引用
吴丽敏,陈立祥,梁丽娟,王筝,王淼,刘少伟,熊云昭,王萱,许庆友..依普利酮下调 SGK-1表达抑制梗阻性肾病细胞增殖的研究[J].中国药理学通报,2016,(1):69-73,5.基金项目
国家自然科学基金资助项目(No 81273684,81473652),河北省自然科学基金资助项目(No H2015423009) （No 81273684,81473652）
