中国药理学通报Issue(1)：79-83,5.DOI:10.3969/j.issn.1001-1978.2016.01.017
显脉旋覆花不同部位体内外抗氧化作用比较
Comparison study on in vitro and in vivo antioxidant activities of Inula nervosa Wall. extracts from different parts
摘要
Abstract
Aim To study the in vitro and in vivo an-tioxidant activity of Inula nervosa wall. in order to le-gitimately use the resources of I. nervosa. Methods The medicinal ingredients of aboveground and under-ground parts of I. nervosa were extracted by different extraction methods. Ultrasonic extractions from differ-ent parts were compared by their in vitro and in vivo antioxidant effects. Results Ultrasound alcohol ex-traction had the highest content of total phenols and fla-vonoids, with the content of total phenolics much high-er than that of total flavonoids. Ultrasound alcohol ex-tractions had very good scavenging effect on DPPH, ABTS and superoxide anion radical, with the extraction from underground part more effective than extraction from aboveground part. Ultrasound alcohol extractions significantly increased the level of catalase ( CAT), superoxide dismutase (SOD), total antioxidant capaci-ty (T-AOC), glutathione peroxidase (GSH-Px) activi-ty and decreased the level of malondialdehyde (MDA) in liver, kidney and serum in drenching aging mice. The antioxidant activity of high concentration of the ex-traction from aerial part was equivalent to that of low concentration of the extraction from underground part. Conclusions Ultrasound alcohol extractions of I. ner-vosa have very good scavenging effect on free radicals, which indicates good antioxidant ability. Antioxidant activity of underground part is much stronger than that of the aboveground part.关键词
显脉旋覆花/不同部位/提取方式/总酚/总黄酮/抗氧化Key words
Inula nervosa Wall./different parts/ex-traction methods/total polyphenol/total flavonoids/antioxidant分类
医药卫生引用本文复制引用
贺安娜,佘朝文,曾军英,彭圣贤..显脉旋覆花不同部位体内外抗氧化作用比较[J].中国药理学通报,2016,(1):79-83,5.基金项目
湖南省科技计划重点项目( No 2013FJ4324) （ No 2013FJ4324）
湖南省科技计划一般项目(No 2013NK4108) （No 2013NK4108）
植物学湖南省“十二五”重点建设学科资金资助(No ZWX2014-4) （No ZWX2014-4）
