中国药理学通报Issue(1)：84-88,89,6.DOI:10.3969/j.issn.1001-1978.2016.01.018
Sorting nexin 10缺失对小鼠巨噬细胞功能的影响
Effects of Sorting nexin 10 deficiency on function of mouse macrophages
摘要
Abstract
Aim To discuss the influence of endosome/ lysosome transport proteins SNX10 on macrophage, providing new potential targets for the treatment of variety of related immune diseases . Methods The genotype of mice was identified by PCR. The role of SNX10 in phagocytosis of bacterial components and sterilization by macrophages were assessed. The levels of TNF-α、IL-12 / 23 p40 and IL-6 were measured by q-PCR and ELISA assay. Finally, the NF-κB signaling pathway was evaluated by Western blot and immuno-fluorescence staining. Results Ex vivo experiments showed that SNX10 knockout could enhance bactericid-al ability and inhibit the expression and production of TNF-α, IL-12 / 23 p40 and IL-6 of macrophages.These effects might attribute to the inhibition of NF-κB signaling pathway activation. Conclusion SNX10 knockout could enhance bactericidal ability and inhibit the inflammatory response of macrophages, and its mechanism may be achieved through the NF-κB signa-ling pathway.关键词
SNX10/巨噬细胞/细胞因子/杀菌/TNF-α/IL-12/23 p40/IL-6/NF-κBKey words
SNX10/macrophage/phagocytosis/ster-ilization/TNF-α/IL-12 / 23p40/IL-6/NF-κB分类
医药卫生引用本文复制引用
李婉贞,游艳,彭锦,周春,李东,沈晓燕..Sorting nexin 10缺失对小鼠巨噬细胞功能的影响[J].中国药理学通报,2016,(1):84-88,89,6.基金项目
国家自然科学基金面上项目(No 81573441,81371923) （No 81573441,81371923）
广东省自然科学基金课题(No S2013010015313) （No S2013010015313）
