中国药理学通报Issue(1)：89-93,5.DOI:10.3969/j.issn.1001-1978.2016.01.019
白藜芦醇通过降低 NF-κB p65乙酰化缓解大鼠神经病理性疼痛
Resveratrol facilitates neuropathic pain in rats model by decreasing acetylation of NF-κB p65
摘要
Abstract
Aim To investigate the antagonistic effect of resveratrol on neuropathic pain and its underlying mechanism. Methods Neuropathic pain was induced by ligation of L5 spinal nerve (SNL) in rats. 90 male Sprague-Dawley rats, fit with intrathecal catheters were divided randomly into six groups ( n = 15 ): naive group; sham group; SNL group; high dosage of res-veratrol group (300μg);middle dosage of resveratrol group ( 30μg ) and low dosage of resveratrol group (3μg). The naive group did not make any process. In sham group, the L5 spinal nerve was only exposed without ligation. Other groups received SNL. Different dosages of resveratrol dissolved in 10μL 100% DMSO were administered by intrathecal injections once a day for 4 days, starting on day 4 after SNL. Paw withdraw-al latency (PWL) was measured on day 1,3,5,7,9, 11,14 days after surgery separately. On day 7 after be-havioral testing, the lumbar segments of the spinal cord were removed to measure the level of SIRT1 and acety-lated-p65(Ac-p65) for western blot. The activation of NF-κB was determined through calculating the percent-age of NF-κB-immunofluorescence positive staining cells in this study. Results Compared with sham groups,the SNL group showed an obvious decrease(P< 0. 05) of PWL and SIRT1 after surgery,whereas Ac-p65 and actived NF-κB significantly increased ( P <0. 05) in the spinal cord. Administration with high and middle dosages of resveratrol markedly attenuated(P <0. 05) SNL-induced thermal hyperalgesia and down-regulation of SIRT1 and blocked (P < 0. 05) the SNL-induced up-regulation of Ac-p65 and actived NF-κB in the spinal cord. Conclusion Intrathecal resveratrol can inhibit the development of neuropathic pain and suppress the activation of NF-κB signaling in SNL rats . The analgesic effect of resveratrol is implemented partly via increasing the level of SIRT1 and deacetylat-ing p65.关键词
神经病理性疼痛/白藜芦醇/沉默信息调节因子 1/NF-κB/p65/乙酰化Key words
neuropathic pain/resveratrol/silent infor-mation regulator 1/NF-kappa B/p65/acetylation分类
医药卫生引用本文复制引用
王依慰,刘清珍,陈春龙,支亦博,章洁,李伟彦..白藜芦醇通过降低 NF-κB p65乙酰化缓解大鼠神经病理性疼痛[J].中国药理学通报,2016,(1):89-93,5.基金项目
全军“十二五”面上资助项目(No CWS11J017) （No CWS11J017）
南京军区医药卫生科研基金资助项目(No 14ZX15) （No 14ZX15）
